Font Size: a A A

The Preliminary Studies On Endophyte Of Brucea Javanica(L.)Merr.

Posted on:2013-09-14Degree:MasterType:Thesis
Country:ChinaCandidate:J XiaFull Text:PDF
GTID:2234330371498253Subject:Pharmacognosy
Abstract/Summary:PDF Full Text Request
It’s shown in the relevant literature that endophytes in many traditional Chinese medicine can produce the same or similar active ingredients with their hosts. In order to better protect and develop the limited wild Chinese medicine resources, many scholars screened many effective strains and the active metabolite to bmicroorganism in special ecological environment has close relations with special secondary metabolites, which can be applied in the microbial fermentation technology to the implementation of the industrialized production of natural drugs.Brucea javanica is the seeds of the plant Brucea javanica(L.)Merr. which belongs to Brucea of Simaroubaceae. It’s a common tradional Qingrejiedu drugs, and it also showed good antibacterial, insecticides efficacy. Pharmacological studies have shown that Brucea javanica oil emulsion was significantly inhibited Helicobacter pylori,therefore it was often used as clinical anti-petic ulcer drugs. The the ethanol extracta of its oil also showed strong inhibition of Staphylococcus aureus and other human pathogens.Based on the theories above, searching the population structure of endophytes in Brucea javanica and their antibacterial activity of secondary metabolites is of great significance to explore new drug source and the secondary development of traditional Chinese medicine.The paper includes several parts:1. The review of domestic and foreign literatureAfter reviewing lots of relevant literature, authors summarized the general research situation of Brucea javanica on the chemical composition, pharmacological effects, clinical application of fromulations and resources. Then we turned to the recent progress of medicinal endophyte,including the biological diversity of endophytes in medicinal plant, screening of the active metabolite,especially antibacterial and antagonism of pathogens.2.The preliminary studies on endophytes of Brucea javanica(L.)Merr.Studies on the biodiversity of endophytes from Brucea javanica:By separating and purifying endophytes from different organs of Brucea javanica again and again,151strains of endophytes were isolated, including92strains of fungi and59strains of endophytic bacteria. The biodiversity is obvious both in the composition of the endophytes species and the viarious distribution in different ogans. The largest number of endophytic fungi,bacteria and actinomycetes were respectively from leaves,sterm and inflorescence. The species of endophyte in fruit is simple and without specificity.The diversity index of endophytic fungi H reaches2.65, indicating a high variety of strains relatively. The distribution of endophytic fungi showed both extensity and the specificity in different tissues from Brucea javanica.Simultaneously, the types of endophytes varied in different plant organs, and the same endogenous strain also had clear differences in distribution within different organizations.92endophytic fungi were morphologically identified belonging to20genera.59endophytic bacteria containing53cocci (14with capsule,39without capsule)4bacillus and2vibrio.Antibacterial experiment of endophyte from Brucea javanica:To investigate the antibacterial activity of the endophytes’fermentation broth, we chosed4kinds of human pathogens as target bacteria, using the filter paper method, screened a total of45active strains, including12showed strong activity.Tissue culture and rapid propapagation research:When using tissue explant method to isolate the endophyte with the sagments of Brucea javanica as explants, like sterm,leaves and inflorescence,we also studied the effects of minimal medium and plant growth regulators on the tissue culture and rapid propapagation. The result showed that the best formula of proliferation, robust seedling and rhizogenesis were MS+6-BA1.0mg-L-1+IBA0.1mg-L-1,1/2MS+banana juice20g-L-1,1/2MS+IBA0.1mg-L-1+NAA0.1mg-L-1, respectively. The survival rate reached the top when using sands as culture medium.
Keywords/Search Tags:Brucea javanica(L.)Merr, Endophyte, Biological diversity, Antibacterialactivity
PDF Full Text Request
Related items