The Quantitative Analysis Of Blood Glucose Using Raman Spectroscopy

Blood glucose is the sugar of blood, which exists as glucose in blood. Every tissue and cellular activities are always in need of energy by glucose. With the supply of energy, all tissue system is possible in normal operation. In order to ensure energy needs of every tissue and cell, blood glucose must be maintained at a certain level. So, what level blood glucose reaches is normal? Blood glucose concentration of healthy man in the morning should be in80-120mg percent before breakfast. When the level blow70mg percent, we call that hypoglycemia and if when the blood glucose concentration is over130mg percent, this phenomenon is known as hyperglycemia. When the blood glucose level exceeds160-180mg percent, there will be a part of glucose with excreted urine, which we call diabetes mellitus. If hungry time is long or sustained vigorous physical activity, hypoglycemia patients appear dizziness, palpitations, sweating and hunger and other reaction. If blood glucose concentration drops continuously to less than45mg percent, there will be hypoglycemia coma risk phenomenon. Disposable or short period of high blood glucose is no serious damage for human body. Excited mood, tense, stress and all can incur transient hyperglycemia situation. Subsequently, it slowly returns to normal level of blood glucose. Long-term hyperglycemia is harm for human. it will incur the organism of the body lesions. For example, pancreatic failure, resistance renal function impairition and other chronic complications. Diabetes mellitus is particularly high and very serious for human life. The light will lead to function problem of the body, serious person may be fatal. Diabetes is a common endocrine and metabolic diseases, it is due to defects of insulin action or the organism endogenous insulin secretion defects, which induce chronic systemic metabolic disease with sustained hyperglycemia. It will bring some of the acute and chronic complications, Such as lactic acidosis, diabetic ketoacidosis, diabetic hyperosmolar syndrome, microvascular complications and so on. Although the disease is very terror to us, we can’t fold our hands. If we found the disease early and took effective treatment measures, we could control the disease. Blood glucose monitoring for those patients with diabetes and hypoglycemia is very important. Through the detection of glucose, they can master the change of their blood sugar levels. The data of blood glucose has guiding significance on their diet, patterns of life and the reasonable use of drugs. When the blood glucose level is not normal, patients also find in time to medical treatment in order to avoid the serious situation. Blood glucose monitoring can also be used to reflect the therapeutic effect, and provides data basis for the adjustment of treatment scheme and the condition of treatment.However, current blood glucose measurement is a invasive or minimally invasive, for the diabetic patients of receiving insulin treatment, they need4-7times blood glucose detection. As for hypoglycemia and other relevant glucose complications also require frequent monitoring of blood glucose to know the blood glucose level. The blood glucose monitoring is so frequent that patients had bear physical pain and mental damage. In this paper, we explore a noninvasive measurement of blood glucose concentration method, in order to further study the clinical noninvasive glucose monitoring method.Raman spectra belong to the molecular vibration spectrum and rotation spectrum derived from molecular vibration and rotation energy level transition. So Raman spectrum could obtain the molecular structure information of the material. The sample can be directly detected nondestructively through the optical fiber probes or glass, quartz, and optical fiber. Raman spectroscopy is high resolution, good reproducibility, simple and rapid and very weak for the Raman scattering of water. So Raman scattering method is particularly suitable for water system, especially for biological specimens and inorganic substance. Raman spectral has clear and sharp peak. The peak intensity and the concentration of active components of measured material are proportional. Accordingly, you can use the Raman spectrum to detect some components of the organism quantitatively.This paper explores a noninvasive measurement of blood glucose method with Raman system. Before the vivo test, we first make a confirmatory test in vitro, which is the analysis of Raman spectroscopy of blood glucose. Blood spectrum of rat is measured systematically in vitro after increase of blood glucose caused by injection of glucose to explore an new analysis method. The peak of blood spectrum is analyzed, we also find the intensity change of1125cm-1/1549cm-1also correspond with the change of blood glucose and the linear relationship reaches094. which shows Raman technique can analyze the content of blood sugar quantitatively and provides theoretical basis for measuring blood glucose non-destructively in vivo. Using mice as a mode, blood spectrum of rat is measured systematically in vivo after increase of blood glucose caused by injection of glucose. The peak of blood spectrum is analyzed, we find the intensity change of1125cm-1/1549cm-1also correspond with the change of blood glucose and they have a good linear relationship which reaches098. Our experiment shows the potentiality in measuring blood glucose non-destructively using Raman technique.