Citric Acid On Apoptosis Of Testicle Tissue Of Mice

Objective: To study the effect of citric acid on mouse testis cell apoptosis anddiscuss the mechanism of mouse testis cell apoptosis, DNA-ladder, determination ofBax, Bcl-2gene transcription and protein expression were detected.Methods：40Department of Kunming male mice were randomly divided into4groups, each group of10. Including control group, citric acid, high dose group, low in.To adapt to the environment after a week of mice, the mice in each group were treatedas follows: control group, intraperitoneal injection of0.2mL saline; citric acid of lowdosage group, middle dose group, high dose group were intraperitoneal injection of180mg/kg abdominal cavity;270mg/kg;360mg/kg citric acid solution, injection doseare0.2mL.1times a week, continuous infusion for3weeks, third times the seventhday after injection with mouse testis correlation detection.Results:（1） through the cell DNA-ladder profiles observed: middle doseand high dose citric acid treatment group from mouse testis cell apoptosis, and citricacid of low dose group did not induce apoptosis of testicular tissue generation.（2）Citric acid can obviously influence the Bax gene transcription and protein expression,while no significant effects of Bcl-2. At the level of gene transcription, citric acid islow, middle dose group control group there was no significant difference between Bax(P>0.05), High dose group and the control group with significant difference（P<0.05）;Compared with the control group, citric acid is low, middle, high dosegroup Bax expression is increased; Citric acid is low, middle, high dose group Bcl-2and control group had no significant difference between (P>0.05). Citric acid in lowdose group and Bax/Bcl-2group and control group had no significant difference(P>0.05), Bax/Bcl-2of middle and high dose group significantly higher than those incontrol group (P<0.05). In protein expression, Citric acid is low, middle, high dosegroup protein expression level of Bax compared with the control group increasedsignificantly (P<0.05); Citric acid is low, middle, high dose group Bcl-2proteinexpression and the control group had no significant difference between (P>0.05), Compared with the control group, high dose group, low, in the expression of Bcl-2protein shows ascendant trend; Citric acid is low, middle, high dose group Bax/Bcl-2compared with the control group increased significantly (P<0.05).（3）The activityof Caspase-3in Citric acid is middle and high dose group was higher than that ofcontrol group (P <0.05), Citric acid low dosage group compared with the controlgroup, no significant difference (P>0.05).Conclutions: A certain dose of citric acid could cause imbalance of the effect ofBax and Bcl-2on apoptosis through regulating of Bax gene transcription and proteinexpression, leading to activation of Caspase-3to induce apoptosis of testis.