Establishment Of Rapid Propagation And Regeneration System From Shoots And Leaves In Vitro Of Cranberry

Cranberry (Vaccinium macrocarpon Ait.) is a fruit tree with high economical value. In order to speed up the propagation of cranberry cultivars, the factors which influence rapid propagation and regeneration from shoots and leaves in vitro were studied by tissue culture. The main results abtained are as followings.1. Shoots collected from December to July were treated with84Disinfection Solution5min or0.1%HgCl23min. The inoculation survival rate was above70%. Improved WPM medium with ZT1.0mg/L was appropriate for initial induction of shoots.2. Improved WPM medium was appropriate for subculture also. The medium with additional of sugar30g/L, ZT0.5mg/L and NAA0.2mg/L, adjusted pH value between4.5and5.0was more appropriate. The length96.30mm and multiplication coefficient4.73of shoots were abtained respectively on the medium. The effect of all ZT treamtments on shoots multiplication coefficient were better than2ip treatments. The shoot height and multiplication coefficient with ZT0.3mg/L treatment were85.5mm and4.3respectively.The shoot height and multiplication coefficient with2ip5mg/L were77.6mm and3.87respectively. The shoot height and multiplication coefficient turned to decrease with the higher or lower concentration of ZT and2ip.TDZ1.0mg/L～5.0mg/L treatment inhibited obviously the enlongation growth and multiplication of cranberry shoots.3. During the subculture, the effect of laying method result showed that foward cutting of shoot section into medium was better than conversed or prostrated cutting.4weeks per subculture cycle was more reasonable.4. As medium for inducing adventitious buds, the Anderson salt+MS organics medium brought about best regeneration rate of85%, which was better than Anderson, Motified WPM,1/2MS and MS medium. Medium with addition of sucrose20g/L, agar7g/L and pH value adjusted between4.8and5.2brought about better regeneration effect.5. The different hormones used in the experiment showed significantly different roles on the effect of induction rate and regeneration rate. Adventitious buds from leaves could be induced with BA, CPPU TDZ, ZT,2ip or BR,6-BA2.0mg/L with regeneration rate of48%, CPPU2.0mg/L with62%, TDZ3.0mg/L with45%, especially BR0.01mg/L with regeneration rate of100%. Combination of2ip1.5mg/L and TDZ2.0mg/L got the adventitious buds regeneration rate to be70%. Treatment of combining ZT0.3mg/L and TDZ2.0mg/L showed the regeneration rate to be60%. Addition of IAA, IBA, NAA and2,4-D could decrease regeneration rate, while would increase induction rate.6. Dark culture was one of the efficient methord for advicious buds regeneration from leaves of cranberry. Dark for15to20days in the trial induced adventitious buds regeneration rate up to83%. Adaxial surface of whole leaf touching the medium got better effect of regerneration than other methord.7. Bottle-rooting treatment with IBA0.5mg/L got rooting rate of86%, NAA0.5mg/L with rate of82%, IAA1.0mg/L with74%. As rooting trail in field, shoots dipped rapidly with IBA1000mg/L or IAA2500mg/L got rooting rate up to100%, NAA1000mg/L with the rooting rate of90%. Moss and sand with the equal volume proportion was selected as appropriate matrix medium for cuting, with the survival rate of up to93%.