The Impact Of Different Micro-organisms On The Number And Structural Characteristics Of Soil Humic Acid And Humin

Soil organic matter (SOM) the level of content is an important symbol of the evaluation of soil fertility is one of the important indicators to measure soil quality. SOM the main humus, humus, including humic substances (HS) and non-humic substances. For long-term culture conditions, different microbial role in HS formation process, the humus to form a modified method of Bacteriological (B1for the Bacillus genus, B2, Bacillus), actinomycetes (A1cysts Streptomyces are, A2, genus Streptomyces, A3Lee color Streptomyces spp.), fungi (F1green Trichoderma spp, F2, F3also detected), and wholeBacteria (Bacteria, Actinomycetes, Fungi and mixed) were inoculated to the addition of organic material of the soil, and inoculated microbial control (CK), a total of five treatments for long-term training, periodic sampling, determination of the number of microorganisms and microbial biomass carbon (SMBC,), soil Humus, humic acid (HA) and humin (Hu), the number of, elements of thermal nature and optical nature, clear the Select the different microbial groups in the long-term culture conditions the formation of HA, fulvic acid (the FA) the number of proportion as well as HA and Hu’s structural characteristics, as the microorganisms in the soil HS formation the role to provide a theoretical basis, which can increase SOM content, through the regulation of the number of microorganisms in order to achieve the sustainable development of soil fertility and carbon sequestration regulation.The results are as follows:(1) Soil microbial quantity has a direct impact on SOC different microbial SOC to reduce the Bacterial degradation of the weakest capacity, the amount of SOC can also affect the number of soil microorganisms and SMBC, Dark Brown and Mollisols, with the prolonged incubation time, the microbial culture soil SMBC and SOC were significantly related.(2) Long-term culture conditions, the DarkBrownSoil and Mollisols, microbial soil C/N can reduce soil soluble Water soluble (WSS), FA and insoluble Hu content decreased early stage of incubation medium solubilityHA content increased, indicating that microorganisms can promote the formation of the HA and the formation of a speed greater than the FA. The most obvious action of Bacteria, Actinomycetes followed by Fungal least.(3) Dark Brown Soil and Mollisols, the structure of HA than FA single microorganisms to promote HA oxidation degree enhanced Bacteria to promote the structure of the HA molecule to the direction of simplification, the degree of condensation of aromatic rings per unit of carbon increases, and Actinomycetes to promote soilthe newly formed HA has a strong aliphatic. Fungi HA, the lowest H/C, indicating that the Fungi to promote the condensation degree of enhancement of the HA, prompting the HA is a high degree of aromatization, cracking energy. Each HA sample strength class shoulder-like absorption peak at280nm, this may be characteristic of lignin. (4) Dark Brown Soil and Mollisols, the microorganisms reduce the amount of the Hu aromatic compounds, prompted Hu degree of oxidation increased. Actinomycetes may promote Hu molecular structure simplification, which is due to the Actinomycetes to promote the decomposition and transformation of organic matter in Hu, while Fungi Hu aliphatic structure and peripheral carboxyl functional groups is very small, can contribute to the aromatic compounds of Hu’s increase in the condensation degree of enhancement, the complex molecular structure.