A Study On Callus Induction And Organogenesis In Acer Mono Maxim

Acer mono Maxim is a very important indigenous forest tree species, with the excellent characters of fast-growing, long-life and nice quality of wood, which is widely used in environment protection, food industry, medicine, industrial production, and the landscape tourism resources. At present, Acer mono Maxim is propagated mainly by seed and stem cutting. However, seed propagation has disadvantage of long cycle period, low propagation coefficient, and prone to mutation. Propagation of Acer mono Maxim by cuttings has lower survival rate, which is the diffculty we have to face with and this also result in the higher price of Acer mono Maxim seedling in the market. Hence, Rapid propagation techniques of Acer mono Maxim become a major problem of trees industry need to solve.In this paper, we used Acer mono Maxim as materials. We mainly to study callus induction and organogenesis in Acer mono Maxim. The main results are as follows:1. As a sterilization material, NaClO can control microbial contamination. We identified the best factors of the explants of Acer mono Maxim:the best sterilization conditions were being soaked in70%alcohol for30s, and being sterilized in0.8%of NaClO for25min.2. In callus induction of Acer mono Maxim, the young leaves is the best explant material. The effects of explant and hormone on Acer mono Maxim callus induction were explant>6-BA>NAA>2,4-D. According to the growth of callus and orthogonal test, the best combination of Acer mono Maxim callus induction was MS+6-BA0.5mg/L+2,4-D1.0mg/L+NAA0.5mg/L.3. In callus proliferation of Acer mono Maxim, the effects of hormone on Acer mono Maxim callus proliferation were6-BA>2,4-D>NAA>TDZ. And the best combination of Acer mono Maxim calius proliferation was MS+6-BA1.5mg/L+2,4-D0.5mg/L+NAA0.5mg/L+TDZ1.5mg/L.4. According to this research, the types and applying amount of the phytohormones have significant effects on the organogenesis of Acer mono Maxim. The optimal proliferation medium for callus was MS+6-BA1.0mg/L+2,4-D0.5mg/L+NAA0.5mg/L+TDZ1.5mg/L. MS+6-BA0.3mg/L+NAA0.05mg/L had effect on adventitious buds induction from callus and appropriate medium for shoot proliferation is MS+TDZ0.04mg/L.