Study On Growth Control Of Salmonella Enterica Choleraesuis On Grass Carp Fillets

Bdellovibrio are predators of other bacteria. It can lyse some common bacteria, for example E.coli, salmonella and so on. Reports on Bdellovibrio sp was successfully used in waterpurification, aquaculture, poultry breed and medical specialty were common. As a kind ofliving creaster decontamination factor，Bdellovibrio has good parasitism, wide host range andno residue. It will be a wise choice of using Bdellovibrios to eliminate or suppress thepathogens in food.The selective medium without organic nutrients was applied and the host bacteria wasthe only prey for the Bdellovibrio to lyse in the study. Then the other bacteria was curbed andthe Bdellovibro was purified. Finally, the purified Bdellovibrio was fermented to expand inthe laboratory and applied in the elimination experiment.In order to control growth of pathogen on the uncooked or half-cooked food like sashimi,effectiveness and availability of physical (HPP), chemical (H2O2) and biological methods(Bdellovibrio bacteriovorus M01) were compared in controlling growth of Salmonella entericCholeraesuis (S.Choleraesuis) on grass carp fillets.The results showed that all of the three methods caused a significantly reduction of thispathogenic bacterium (p<0.05); In the lysis experiment, the effect of biological method is thebest and the low-dose group; In the sensory assessment, biological method was better thanphysical and chemical methods in all the48hours and ensure sensory attribute of the fillets.During storage at25℃, although with a higher original bacterium population than the othertwo methods, BDM01performed better in controlling bacterial growth. The results indicatedBALO (Bdellovibrio-and-like organisms) possess potential application value in fillets storagewith low contamination.