The Location Of Gout Susceptibility Gene On Chromosome 4q25.

Objective:Tai Wan scholoars and our group have all found that the chromosome 4q25 region is strongly associated to primary gout. Our study will use DNA chips to locate the single nucleotide polymorphism of primary gout and conform the relation ship between them.Method:We included 480 cases of primary gout patients,480 healthy male controls. DNA was extracted from peripheral blood by QIAGEN DNA kit. We found the tagSNPs from Hapmap database and used Hapviewer software to check the Hardy-Weinberg equilibrium of the SNPs to insure the study randomness. Golden Gate DNA chip was performed to genotype the alleles selected before. Genotype frequencies between groups were compared byχ2 test to identificate related polymorphisms on chromosome 4q25 region with gout to identify the SNPs associated with primate gout.Result:1. Analysis of chromosome 4q25 gene sequenceAccording to hapmap database and the results of the haploview software, we ultimately selected 96 tagSNPs (the minimum allele frequency should be larger than 0.05 and smaller than 0.8).2. Genotype frequencies in cases and controlsAccording to the analysis of DNA chips'results, all the chip call rates are 99.9%. There are 4 genes which each have a tagSNP associated with gout.According to the Bonferroni correction principle, the P value of 96 DNA chips shouldbe less than 5.2* 10-4. After correction, we determined that the rs12504538 on ELOVL6 gene was associated with primate gout.ConclusionThe rs12504538 on ELOVL6 is associated with primate gout in Chinese han people. We need amplify more cases and function study to vertify the relationship between rs12504538 and primate gout.