| Objective:To establish two kinds of rat intervertebral disc degeneration models by anulus puncture and lumbar instability,and to compare their respectively of characteristics by using histopathology and radiological methods. Method :Fifty-four healthy SD rats were randomly divided into 3 groups: the control group,the puncture group and the lumbar instability group.Every group had 16 SD rats.In the puncture group,intervertebral disc of L3,4,L4,5 and L5,6 were exposed by posterolateral abdominal approach using a 21-gauge needle to make a full-thickness puncture.Puncture depth is 2.3mm.The lumbar instability group rats were resected the spinous process,interspinous ligament,supraspinous ligament and zygapophysis of L3L6 vertebra through back median approach.Taked 6 SD rats each group to examine their lumbar intervertebral disc at 2,4,and 8 weeks after operation with X-ray,MRI and histopathology methods.Result:In the puncture group observed following signs:the height of intervertebral space narrowing,lumbar curvature smaller,annulus rupture,T2-weighted image declined at week 2 after operation.The degeneration signs was increased at week 4 after operation.At week 8 after operation observed following signs:intervertebral space appeared the change of wedge shape,vertebral edge formed osteophyte,spondylolisthesis,nucleus appeared shrinkage and fibrosis,annulus appeared large number of radial cracks.In the lumbar instability group,signs of degeneration appeared at week 4 after operation.Degeneration was slowly progressed at week 8 after operation.Compared with the control group,the puncture group and the lumbar instability group severally occured significant difference with MRI scores at week 8 after operation(P<0.05).Conclusion:The rat model of intervertebral disc degeneration can be successfully constructed by the methods of anulus puncture and lumbar instability.The degenerative process induced by anulus puncture is faster and more severe than the induced by lumbar instability. |
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