| Carapax Trionycis,as a traditional chinese medicine, originated from the shell of Trionyx sinensis Wiegmann. It has such activities as replenishing "yin", restraining "yang", softening and resolving hard masses,reducing fever and removing steam; It is used for anti-fever, windy syndrome agents, abdominal mass. Crude Carapax Trionycis have a firm textrue and putrid odor. It has such activities as replenishing the vital essence and removing heat, restraining "yang" and extinguish wind. It is used for replenishing "yin" hurted by febrile diseases, internal dampness and endogenous collateral wind. Processed Carapax Trionycis is better at resolve hard mass.It is usually used for abdominal mass, hot flashes from "yin" deficiency and menopause. After being processed, the frim texture gets loosen and could be easily smashed; Processing can also increase extract efficiency, modify odour and taste. Vinegar processing have effects on convergence, detoxification, scattered silt convalescence and alleviate pain; It can also strengthen the effect of drugs being taken up by liver channel, removing food retention,softening and resolving hard masses of liver. Under the guidances of traditional chinese medicine theory,with multidisciplinary methods and modern analysis technology, this treatise reported comparative research results on pHarmacological effect, chemical composition and the quality analysis of pre-and-post-processed Carapax Trionycis.Comparative study on influence of pre-and-post-processed Trionyx sinensis carapace on hepatic stellate cell proliferation(HSC-T6) was taken using the method of ultrasonic extraction and reflux extraction,and we choose processed Trionyx sinensis carapace reflux extraction as our optimized method. Then membrane dialysis was used to divide the extracts of Carapax Trionycis into different molecular weight portions,after that we applied pHarmacological assays as HSC-T6 proliferation assay to detect the impacts of Trionyx sinensis carapace extracts at different concentrations on HSC-T6,and found the active anti-hepatic fibrosis principle was determined to be portion cantaining compounds molecular weight from 3000 to 4000.On the basis of the fingerprint of the active principle of Carapax Trionycis by HPCE has been formulated,we established a fingerprint method for comparing the same geograpHical origin of pre-and-post-processed carapace Trionycis in anti-liver fibrosis,10 crude samples and 10 vinegar-processed samples were analyzed. Seventeen co-possessing peaks were selected as the fingerprints of 10 crude samples,the similarity of the fingerprint were above 0.94; Nineteen co-possessing peaks were selected as the fingerprints of 10 vinegar-processed samples, the similarity of the fingerprint were above 0.93. Trough the comparison of fingerprints of pre-and-post-processed Carapax Trionycis and the attribution of active anti-hepatic fibrosis principle separeted for the extracts,we found that the content of Trionyx sinensis carapace appears to change greatly before and after vinegar processing, and after processing some new compounds emerged. It is the first that we used electropHoresis method (SDS-PAGE) to idetificate carapace Trionycis pre-and-post-processed,and the moleculer weights of the main peptides were analysised too. With modern analysis technology, systematic researches on origin, character, microscopical identification, extracts(water-soluble contents, ethanol-soluble contents), the content of water,ash, acid-insoluble ash, and the UV-Vis analysis methods of the content of amino acids and peptide of pre-and-post-processed Carapax Trionycis were carried out. Above that, we established a relatively perfect standard for controling the quality of pre-and-post-processed Carapax Trionycis, with which can effectively control the quality of Carapax Trionycis,it can also provides a reference for the new edition of "Chinese PHarmacopoeia" amendment of Carapax Trionycis quality standards. |
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