The Impact Of HBV On Oval Cells And Its Effects On The Expression Of P8

Objective:To investigate the impact of HBV on hepatic oval cells and liver cells.Methods: Hepatic oval cells and liver cells were isolated from adult mice and HBV Transgenic mice. The liver oval cells and liver cells are characterized by staining of glycogen and Immunofluorescence.Results: It is possible to isolate hepatic oval cells and liver cells from adult mice and HBV Transgenic mice. Isolated cells were defined as oval cells or liver cells by a6-pkm2,afp-hnf-4,ck19-alb.Conclusions:It is possible to isolate hepatic oval cells from adult mice and HBV Transgenic mice. Objective:To investigate the impacts of HBV on the expression of P8 in liver cells and oval cells and variation in human liver tissue, and analyze the clinical significance.Methods:The expressions of P8were detected by real time- PCR in oval cells and liver cells between two groups. The expressions of P8varied in human liver tissue with no HBV , human liver tissue with HBV and liver cancer with HBV.Results:The expressions of P8in liver cells and oval cells from HBV Transgenic mice was lower than that from adult mice(P<0.05). The expressions of P8in human liver tissue with HBV and liver cancer with HBV was lower than that in human liver tissue with no HBV(P<0.05).Conclusions: HBV may induced hepatocarcinogenesis through its impact on liver cells and oval cells ,and can depress the expressions of P8.