The Research On Chemical Composition Of The Fresh Fruit Of Physalis Alkekengil. Var. Francheti And Harmonia Axyridis

Phytochemical investigation of the fresh fruit of Physalis alkekengi L. var. franchetiMast.) Makin (Solanaceae), led to the isolation and structural elucidation of a newphysalin (5a,6b-dihydroxyphysalin C) and nineteen known compounds. Their structureswere identified as: Linoleic acid (1), 2-((2-ethylhex- yloxy)carbonyl) benzoic acid (2), b-Sitosterol (3), Butanedioic anhydride (5), Citric acid (6), b-Daucosterol (7), D-glucose (8),9,12-Octadecadienoic acid ethyl ester (10), Physalin A (11), 1-O-b-D-glucopyra- nosyl-N-(2′-hydroxypalmitoyl) octadecasphinga-4,8-dienine (12), Physalin D (14), Succinicacid (16), 4,7-didehydrophysalin B (17), 1-Citric acid ethyl ester (18), 1-Citric acidmethyl ester (19), 4,7-didehydroneophysalin B (20), 5a,6b-dihydroxyphysalin C (21),saccharose (22), 5-(hydroxylmethyl)furan-2-carbald-ehyde (24) and cyclo-(L-leuc- yl-Lisoleucyl)(26), via spectroscopic tests (HRESI-MS, GC-MS, IR, UV-VIS, 1D and 2DHMR). On the basis of (DPPH?) assay, the anti-oxidant activities of compound 4, 11, 12,14, 17, 21 were investigated. 11 showed appreciable anti-oxidant activity.Investigation of Harmonia axyridis (family Coccinella), also known as Ladybird, ledthe isolation and structural elucidation of eight known compounds. Their structureswere identified as: Octadecanoic acid (31), Cholesterol (32), Dibutyl phthalate (33),Phenylalanine (38), Leucine (39), Isoleucine (40), Valin (41) and Alanine (42), viaspectroscopic tests (HRESI-MS, GC-MS, IR, UV-VIS, 1D and 2D HMR).