Expression And Significance Of β-Catenin And E-Cadherin On Ovarian Serous Carcinoma And Serous Borderline Tumor

Objective:Exploreβ-Catenin,E-Cadherin,P53 and Ki-67 protein expression with ovarian serous carcinoma (OSC) and ovarian serous borderline tumours (OSBT) with the pathogenesis of correlation and ovarian serous carcinoma infiltrating and ovary lymph node metastasis relationship.Methods:(1)Choose OSC 42 cases, including high grade serous carcinoma(HGSC)35 cases, low grade serous carcinoma(LGSC)7 cases, clear cell carcinoma(CCC)7 cases, OSBT 18 cases, ovarian serous adenoma(OSA)10 cases and normal ovarian tissue(NOT)5 cases, all of the paraffin embedding organizations;(2) using immunohistochemistry SP method in (3-Catenin,E-Cadherin,P53 and Ki-67 protein expression;(3) Statistical methods:statistical methods 17.0 statistical software SPSS statistical analysis,using X2 inspection, Fisher accurate Spearman rank probability analysis and correlation analysis, analysis the protein expression and histological grade, OSC invasion and lymph node metastasis relationship.Results:1. The expression ofβ-Catenin in ovarian cancer than NOT,OSA,OSBT and OSC four groups overall increased significantly, the expression between the difference was statistically significant (X2=22.736,P<0.05). The expression ofβ-Catenin in NOT, OSBT OSA, compared with opposite OSC groups was statistically significant difference (P<0.05), and NOT the expression differences with OSA was NOT statistically significant (P>0.05).β-Catenin expression with the surrounding tissue infiltration, lymph node metastasis without obvious (P>0.05) correlation;β-Catenin expression with malignant degree rise in ovarian cancer is increasing gradually and the positive expression rate is highest, are statistically significant difference (P<0.05).2. The expression of E-Cadherin in NOT,OSA,OSBT the differences are significant increases in a statistically significant (x2=16.357,P<0.05). The expression of E-Cadherin in NOT, OSBT and OSA three groups gradually increasing (P<0.05), and the expression of E-Cadherin between the two groups OSBT and OSC have lower trend but NOT statistically significant difference (P>0.05). The expression of E-Cadherin with the surrounding tissue infiltration, lymph node metastasis without obvious (P>0.05) correlation, The expression of E-Cadherin in OSC infiltrating groups in negative rate higher than no infiltrating groups have two trend, but group compared not statistically significant difference (P>0.05).3.The expression of P53 in NOT,OSA,OSBT,OSC and CCC five groups, the differences are significant increases in a statistically significant (x2=77.088, P<0.05). The expression of P53 in NOT, OSBT OSA, compared with opposite OSC groups was statistically significant difference (P<0.05), and NOT the expression differences with OSA was NOT statistically significant (P>0.05).The expression of P53 in OSC obviously higher than in CCC expression and the difference was statistically significant (P<0.05). The expression of P53 in LGSC and HGSC, the difference obviously lower than a statistically significant(P<0.05). The expression of P53 with the surrounding tissue infiltration, lymph node metastasis without obvious(P>0.05)correlation; In the positive OSC P53 expression rate is highest, the difference was statistically significant (P<0.05).4.Ki-67 protein expression in NOT, OS A, OSBT and OSC four groups was statistically significant difference compared (F=49.894,P=0.000). Ki-67 protein OSC and CCC in a statistically significant differences between expression (F=-4.591, P=0.000); In LGSC Ki-67 protein expression differences between with HGSC was statistically significant (F=-4.916, P= 0.000).Conclusions:1.The expression ratio ofβ-Catenin in OSA, OSBT and OSC increased, indicating that the expression ofβ-Catenin on the evolution of OST.2.E-Cadherin protein from NOT to the OSA and OSBT gradually increased, indicating that E-Cadherin may have formed to promote the role of OST,and then to OSC from OSBT reduction trend, indicating decreasing trend with E-Cadherin invasion and metastasis of OSC.3.The strong positive expression of P53 and the Ki-67 expression of the LGSC and HGSC, HGSC and differential diagnosis of CCC reference value; P53 strong expression and high expression of Ki-67 may be more meaningful differential diagnosis HGSC molecular targets.