Expression Of Special AT-rich Sequence-Binding Protein1 (SATB1) In Clear Cell Rencal Cell Carcinoma

BackgroundRCC, which accounts for 2% to 3% of all adult malignant neoplasms,of which 60% to 85% is Clear Cell Renal Cell carcinoma,is one of the most lethal of the urologic cancers. Traditionally, more than 40% of patients with RCC have died of their cancer, in contrast with the 20% mortality rates associated with prostate and bladder carcinomas. Approximately 31,000 new diagnoses of RCC are made each year in the United States, and 11,900 patients die of disease. Overall,8.9 new cases are diagnosed per 100,000 population per year, with a male-to-female predominance of 3:2 (China reported the ratio is 2:1). This is primarily a disease of the elderly patient, with typical presentation in the sixth and seventh decades of life. The incidence of RCC has increased recent years,largely related to the more prevalent use of ultrasonography and CT scan for the evaluation of a variety of abdominal or gastrointestinal complaints. Overseas study found that with the methods of examination and treatment more advanced and in time, However,There was a steadily increasing in mortality rate of RCC, and this was observed in all ethnic and both sex groups. Therefore discuss the occurrence and development of the mechanism of RCC have a important value in prediction, early diagnosis, progression, metastatic potential and prognosis of this diseases.Special AT rich sequence binding protein (SATB1) was first obtained by Dickinson etc in 1992, using immune globulin heavy-chain muon chain gene enhance the son 3'MARs sequence as probes, from human cDNA library. In recent years, molecular biology and clinical study found that SATB1 Participate in gene regulation in various ways, Which plays an important role in immune adjustment, apoptosis regulation and tumor metastasis. Some study confirms that the expression level of SATB1 highly related with cancer grading, stages and disease outcomes in breast cancer cells nuclear. Other research has shown that SATB1 may be concerned with the occurance, progression and metastasis of epithelial oarian cancer and associated with the development and lymph node metastasis of NSCLC. Currently there is no reports related to RCC.ObjectiveTo study the expression of AT-rich sequence-binding protein 1 (SATB1) in Clear Cell Renal Cell Carcinoma(ccRCC) and explore the role of SATB1 in the occurrence, progression and metastasis of ccRCC.MethodsRT-PCR was performed to detect the expression of SATB1 in 30 cases of ccRCC tissue and the adjacent non-tumor renal tissue,5 cases of normal renal tissue as well as renal cell carcinoma cell line 786-0 respectively.ResultsThe expression of STAB1 mRNA in ccRCC tissues was significantly higher than that in adjacent non-tumor renal tissues (P<0.05). Weak expression was detected in normal renal tissues. The expression.of SATB1 mRNA was in line with ccRCC stage and was statistically stronger in metastatic renal cell cancer tissues than nonmetastatic ones(P<0.05).ConclusionThe expression of SATB1 may be associated with the occurrence, progression and metastasis of ccRCC.