| Purpose: To establish AchR fragment of 129 to 145 classic animal model of myasthenia gravis - experimental autoimmune myasthenia gravis (Experimental autoimmune myasthenia gravis-EAMG) in rats,the use of"wen yang bu qi"acupuncture therapy on serum acetylcholine EAMG receptor antibody (Acetylcholine receptors antibodies AchR-ab) and interferon-γ(IFN-γ) of the study,confirmed that"wen yang bu qi"acupuncture therapy treatment of EAMG rats and the intervention mechanism. In the treatment of atrophy and further enriches the theory,for the treatment of autoimmune diseases,new methods.Methods: Lewis rats were selected, the AchR fragment of 129 to 145 ratio of 1:2 emulsion with CFA fully established rat model of EAMG. The antigen emulsion to 200μl/only four foot pad injected rats Ministry (50μl), the amount of antigen to 50μg/only. After the first immunization,respectively,4 weeks,8 weeks,again with the method of immuni- zation. Recorded body weight of rats,the reference standard evaluation score Lennon symptoms rating,RNS attenuation expression and serum levels of AchR-ab observation model is successful. Successful model selected 30 rats were randomly divided into model group,pyridostigmine treatment group Ming,"wen yang bu qi"acupuncture therapy treatment group.Keeping with the conditions of the control group over the same period, arrests and treatment under the same conditions; model group is keeping the same conditions over the same period,not to any treatment;"wen yang bu qi"acupuncture therapy group was given"wen yang bu qi"acupuncture therapy group treatment ; pyridostigmine were given pyridostigmine Ming Ming treatment. Respectively on day 1 after onset and intervention (treatment) 14 days after the application of enzyme-linked immunosorbent assay (ELISA) and serum AchR-ab expression of IFN-γlevels,and observed symptoms and weight change per week.Results: 70 rats to model,and succeeded in 32 (death 2) only,the success rate of 45.71%. Observations are as follows: 1. EAMG rats gained weight slowly,appear the more obvious symptoms of myasthenia gravis,clinical symptom score was 1.85,with the control group had significant difference (p <0.05); 2.the expression of AchR-ab serum levels 0.2199 and 0.0357 compared the control group significantly increased,3.serum levels of IFN-γ expression 0.075 220.65 compared with the control group was significantly higher; were statistically significant.Different methods in intervention (treatment),the "yang qi" needling method for treatment group and pyridostigmine treatment group and the next model group compared with rats Lennon method symptom score decreased significantly increased body weight,serum AchR-ab reduce the level of expression,IFN-γexpression was significantly decreased,RNS decay rate decreased;"wen yang bu qi"acupuncture therapy group and pyridostigmine treatment group clearly observed no significant differences;"wen yang bu qi"acupuncture therapy group weight score was significantly better than the pyridostigmine group Ming (p<0.05); IFN-γlevels and serum levels of AchR-ab no significant difference between the two groups.Conclusion: 1. AchR fragment of the experimental application of 129 to 145 with complete Freund's adjuvant immunization in rats,the results of experimental rat and human changes in clinical symptoms similar to MG,Lennon's rating criteria to evaluate symptoms of grade,RNS attenuation expression and serum levels of AchR-ab test, a preliminary model to determine the success EAMG.; 2."wen yang bu qi"acupuncture therapy Treatment of EAMG effective. EAMG rats Lennon method can make the symptoms scores , scores were significantly improved body weight,muscle strength increased significantly improved clinical symptoms; serum AchR-ab expression was significantly decreased; IFN-γexpression was significantly reduced;3. 14 days of treatment,"wen yang bu qi"acupuncture therapy group and pyridostigmine treatment group had significant effect tomorrow,but the score of EAMG rats of body weight differences between the two,"wen yang bu qi"acupuncture therapy group than pyridostigmine next group. |
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