| Purpose: To establish AchR fragment of 129 to 145 classic animal model of myasthenia gravis - experimental autoimmune myasthenia gravis (Experimental autoimmune myasthenia gravis-EAMG) in rats, through the"wen yang bu qi"acupuncture therapy for low frequency repetitive EAMG rats electrical nerve stimulation (repetitive nerve stimulation,RNS) and serum acetylcholine receptor (Acetylcholine receptors AchR) of the study on the"wen yang bu qi"acupuncture therapy treatment of EAMG rats and its mechanism. In the treatment of atrophy and further enriches the theory,for the treatment of autoimmune diseases and the mechanism of providing a new tool.Methods: Lewis rats were selected,the AchR fragment of 129 to 145 ratio of 1:2 emulsion with CFA fully established rat model of EAMG. The antigen emulsion to 200μl / only four foot pad injected rats Ministry (50μl),the amount of antigen to 50μg / only. After the first immunization,respectively,4 weeks,8 weeks,again with the method of immunization. Recorded body weight of rats,the reference standard evaluation Lennon symptoms symptom scores level,RNS attenuation expression and serum levels of AchR-ab to determine whether the model successfully. Successful model selected 30 rats were randomly divided into model group,pyridostigmine out group,"wen yang bu qi"acupuncture therapy group. Keeping with the conditions of the control group over the same period,arrests and treatment under the same conditions; model group is keeping the same conditions over the same period, not to any treatment;"wen yang bu qi"acupuncture therapy group was given"wen yang bu qi"acupuncture therapy group treatment ; pyridostigmine were given pyridostigmine Ming Ming treatment. Respectively on day 1 after onset and 14 days after the application of intervention Oxford-EMG evoked potential (Affiliated Hospital of Changchun University of Traditional Chinese Medicine) detection RNS attenuation change; enzymelinked immunosorbent assay (ELISA) detected the expression of AchR levels,and observe a week EAMG symptoms and weight changes in rats.Results: 1. Application of 70 rats to model success 32 (after the success of Death 2), the success rate of 45.71%. 2.EAMG slow weight gain in rats,showing obvious symptoms of myasthenia gravis, clinical score 2.150, and the control group had significant difference (p <0.05). RNS attenuation test 19.853 and control group 0.064 was significantly higher compared; serum AchR expression 4.044 and the control group 30.746 decreased significantly compared with a statistically significant. 3. Application"wen yang bu qi"acupuncture therapy neostigmine and pyridostigmine treatment,"wen yang bu qi"acupuncture therapy group and the next group of pyridostigmine compared with model group,rats Lennon symptom score decreased body weight increased significantly,RNS attenuation value decreased significantly,Significantly serum levels of AchR expression. 4. In terms of weight gain,"wen yang bu qi"acupuncture therapy group was significantly higher than the result of Ming pyridostigmine treatment group (p <0.05); The weight of "yang qi"group is heavier then serum group.Conclusion: 1. AchR fragment of the experimental application of 129 to 145 with complete Freund's adjuvant (CFA) immunization in rats,the results of experimental rat and human changes in clinical symptoms similar to MG,Lennon's rating criteria to evaluate symptoms of grade, RNS attenuation expression and serum levels of AchR-ab EAMG model to determine the initial success.2."wen yang bu qi"acupuncture therapy treatment had significant effect on EAMG. Can make the weight of rats significantly increased EAMG; Lennon symptom score decreased,muscle strength increased significantly; RNS attenuation values were significantly reduced; significantly increased serum levels of AchR expression.3. 14 days of treatment,"wen yang bu qi"acupuncture therapy group and the next group of pyridostigmine efficacy rather, no significant difference (p> 0.05). However, body weight is relatively EAMG rats,"wen yang bu qi"acupuncture therapy group body weight than pyridostigmine next group,but not statistically significant. |
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