Study On Effect Characteristics And Possible Mechanism Of Weight Reduction Treated By Electro-acupuncture Combined With Sibutramine

Objective:To observe the effects of electro-acupuncture (EA) at ST36 and ST45,sibutramine and EA Combined with sibutramine treating simple obesity rats, and influence on glucose-sensing (GS) neurons in the lateralhypothalamic area(LHA) and ventromedial nucleus(VMN) and expression of Glucokinase(GK) and AMP-activated protein kinase (AMPK); to explore the effect characteristics and the possible central mechanisms of Acupuncture Combined with Western Medicine Treating simple obesity.Method:(1)To establish diet-induced obesity (DIO) rat model by feeding the animals with high fat diet for 12 weeks. Rats were randomly divided into 6 groups:(1) normal control group,(2) DIO model group (3)EA group, (4) sibutramine group, (5) EA Combined with sibutramine group and (6) diet resistance (DR) group.Treatments were administered everyday,resting one day per week. Body weight was measured daily for 5 weeks.After treatments, expression of Glucokinase and AMP-activated protein kinase were mersured by real -time quantity PCR. (1)To make diet-induced obesity (DIO) rat model by feeding the animals with high fat diet for 12 weeks. Rats were randomly divided into 4 groups:(1) normal control group,(2) DIO model group (3)EA group, (4) EA Combined with sibutramine group.After treatments, spikes frequency of GI neurons were recorded.Result:(1)During the course of treatment,after 3 weeks, Compared with DIO model group,the rats weight of sibutramine group and EA Combined with sibutramine group reduced significantly (p=0.022,0.025),but EA group (p=0.113) did not. After 5 weeks,compared with DIO model group,the rats weight and epididymis fat amout of EA group,sibutramine group and EA Combined with sibutramine group reduced significantly (p=0.022,0.025).It indicated that the treatments of simple EA, sibutramine and EA Combined with sibutramine could effectively reduce body weight and quantity of fat; EA Combined with sibutramine treatment produced effects quickly and stable which was better than simple EA and simple sibutramine. (2)The expression of AMPK mRNA and GK mRNA in hypothalamus of DIO rats were lower than normal rats. After the treatment of EA Combined with sibutramine,they were higher than before in rat's hypothalamus. (3)The frequency and glucose sensity of action potential of GI(glucose-inhibited) neurons in DIO group increased,and it shows statistically significant difference,comparing with the normal group(P<0.05).Compared with DIO group,The frequency and glucose sensity of action potential of GI(glucose-inhibited) neurons in EA Combined with sibutramine reduced snd showd statistically significant difference(P<0.05).Conclution:EA at ST36 and ST25 together with sibutramine can reduce body weight,fat and activities of GI neurons effctively,increase the expression of AMPK mRNA and GK mRNA in hypothalamus which may supress the rats appetite.These may be the possible central mechanisms of EA Combined with sibutramine in treating simple obesity.