Effect Of MicroRNA-10b On Invasion Of Choriocarcinoma Cell Line JEG-3

The establishment and maintainance of the normal pregnancy depend on the moderate invasiveness of the embryo trophoblastic cells. In the process of pregnancy, the embryo trophoblastic cells invade into the deciduomata and the helicine arteries, which substitute the vascular endothelial cell. In this way, the uterus helicine arteries are recasted and mother nuclide/fetal interactive circulations are built to provide nutrition for the embryo. The invasiveness descent and invasion failure of the trophoblastic cells maybe result in the recast disturbance of the uterus helicine arteries, which is the most important pathophysiology mechanism of the preeclampsia occurrence.MicroRNA is a kind of non-encoding micromolecule RNA about 19-25 nucleotides length. Through completely or incompletely mating partners with the target gene 3'UTR, the target gene mRNAs are degraded or translations of them are suppressed, thereby the microRNAs participate in regulating vital moment such as ontogeny, apoptosis, proliferation and differentiation etc. There are recent researches indicate that miR-10b which belongs to HOXD gene family is significantly correlated with the cell metastasis and invasion. In the process of embryonic development, it is the one of the important reasons of the preeclampsia that the trophoblastic cells invade the myometrium excessively shallow.Our prior research found that the miR-10b low express pathologically in the preeclampsia period. So we presumed that the miR-10b unbalanced expression maybe participates the preeclampsia pathophysiology process. In this research, we use transient transfection and Transwell chamber experiment to study the correlation of the expression level of the miRNA-10b with the invasiveness of the trophoblastic cells in vitro which is meant to investigate the effect of the miRNA-10b upon the trophoblastic cell diseasesObjectives:To investigate the effect of human microRNA-10B (miR-10b) on the invasion of Choriocarcinoma cell line JEG-3.Methods:The mature type human miR-10b was synthesized chemically. The synthesized miR-10b (20μmol/ml) was transfected into JEG-3 cells. Cells were also transfected with empty vectors and unrelated fragment to serve as control. The expression of mature type miR-10b was detected by Semi-quantitative PCR. The invasion of JEG-3 cells was investigated by Transwell assay.Results:Semi-quantitative PCR showed that cells transfected with mature type miR-10b had significantly higher expression of miR-10b. MiR-10b promoted the invasion of JEG-3 cells respectively.Conclusion:MiR-10b promotes the invasion of the JEG-3.