Study On The Correlation Between The Expression Of Heparanase And The Invasion, Metastasis Of Choriocarcinoma

Objective: (1) To investigate the association between the expression of heparanase and the invasion,metastasis of choriocarcinoma by study -ing the expression of heparanase (Hpa) in human choriocarcinoma cell lines JEG-3 and JAR and human chorionic villitissues. (2) To provide the evidence of heparanase inhibition and heparanase antisense oligodexynucleotides (Hpa ASODN) to control the invasion and metastasis of choriocarcinoma by examining the expression of Hpa in human choriocarcinoma cell lines, compring with human chorionic villitissues.Methods: (1) Matrigel invasion assays were used to show the difference of in vitro invasive ability between JEG-3 cells and JAR cells; (2) Expression of Hpa protein in the human chorionic villi tissues and choriocarcinoma cell lines(JEG-3 cells and JAR cells) were detected by immunocytochemistry and western blot.RESULT: (1) The invasive cell number was significantly larger in JEG-3 cells than in JAR cells(191±16.733vs106±12.572 P<0.05); (2) Hpa protein mainly located in cytoplasm by immunocytochemistry; (3) Protein expression of Hpa was significantly stronger (P<0.05) in choriocarcinoma cell lines than in the human chorionic villitissues by western blot. The Hpa protein expression in JEG-3 cells was stronger (P<0.05) than in the JAR cells ; (4) The data suggested that there were significantly positive correlations between Hpa and the invasiveness of choriocarcinoma cells. CONCLUSIONS: (1) The invasion of JEG-3 cells was stronger than JAR cells; (2) Protein expression of Hpa was significantly stronger in choriocarcinoma cell lines than in the human chorionic villitissues; (3) Activation of Hpa enhance the invasion capability of choriocarcinoma.