Effects Of Different Load Swimming On Nonenzymatic Glycosylation In Aged Mice Induced By D-galactose

Objective:For the current anti-aging research,by focusing on domestic and foreign anti-aging movement and non-enzymatic glycation of the literature, giving a preliminary understanding of the relationships between them. In this animal experiments, through the establishment of D-galactose aging rats animal model and giving different loads swimming, exercise as a means of intervention in rat. By testing AGEs of rats'skeletal muscle ,lung, liver, heart and serum, aiming for the movement of anti-aging theory and research to provide scientific experimental basis.Methods:Experimentâ… : once swimming training on D-galactose aging rats. 40 healthy male SD rats were randomly divided into 4 groups: sedentary control group (SA) 10 rats, D-galactose control group (YA) 10 rats, D-galactose 45min swimming training group (YT) 10 rats, D-galactose 90min swimming training group (YH) 10 rats; in accordance with the experimental design of the experiment rats were D-galactose on modeling, modeling time is eight weeks, when modeling D- galactose induced aging group of regular daily injection of D-galactose solution (125mg/kg body weight), control group injected dose of saline. After modeling, one drawn immediately after exercise for the determination of AGEs, MDA, GSH, CAT, SOD levels or activity of the rat heart, lung, liver, skeletal muscle, serum.Experimentâ…¡: four weeks of swimming training on D-galactose aging rats. 40 healthy male SD rats were randomly divided into 4 groups: sedentary control group (SAF) 10 rats, D-galactose control group (YAF) 10 rats, D-galactose 45min swimming training group (YTF) 10 rats, D-galactose 90min swimming training group (YHF) 10 rats; in accordance with the experimental design of the experiment rats were D-galactose modeling (specific methods with the experimental one.) After modeling, swimming training for four weeks, final harvested after 24 hours of training for the determination of AGEs, MDA, GSH, CAT, SOD levels or activity of the rat heart, lung, liver, skeletal muscle, serum.Results:Experimentâ… ,1. After once swim training, the weight of rats in each group there is no regular change between (P> 0.05); 2.MDA change, heart, liver, skeletal muscle groups were tested YH> YA group> YT group order serum, lung YA group higher than the YT, YH group of five organizations in the SA group were the lowest, and the heart and the YA group P <0.05, compared serum and YA P <0.05; 3.CAT changes heart, lung, liver, YT group> YA group> YH group, skeletal muscle YH, YT group than YA group (P> 0.05); 4.SOD changes, lung, muscle and serum SOD activity in the YT group were tested > YA Group> YH group, lung, skeletal muscle in the YT group and the YH group has significant difference (P <0.05), heart YA group and YH, YT group had significantly lower and the difference (P <0.05); 5 . GSH changes, heart, skeletal muscle, serum GSH levels were tested in the YT group> YH group> YA group, and the heart, serum YT group and the YA group had significant changes (P <0.05), lung, liver GSH content were tested YT group> YA group> YH group (P> 0.05); 6.AGEs change, heart, lung, liver, skeletal muscle content of AGEs YT, YH group were lower than the YA, and the lung, liver, YT compared with the YA group had significantly (P <0.05), serum AGEs levels were YT group> YA group> YH group (P> 0.05).Experimentâ…¡, 1. After four weeks of training, the weight of rats in each group was YTF group> YHF group> YAF group (P> 0.05); 2.MDA change, heart, lung, skeletal muscle, serum MDA content of YTF, YHF groups are less than the YAF group, and in the lung compared with YHF group there was significant difference (P <0.05), liver MDA content was YHF or group> YAF group> YTF (P> 0.05); 3.CAT change, heart, lung , in skeletal muscle CAT activity YTF, YHF YAF group than the high group, CAT activity in liver was YHF group> YAF group> YTF group (P> 0.05), serum CAT activity YTF, YHF group than the YAF group lower (P> 0.05); 4.SOD change, heart, lung, skeletal muscle, serum SOD activity YTF, YHF group than the YAF group is high, and the lungs YHF group and YAF group significant difference (P <0.05) and liver SOD activity was YTF group> YAF group> YHF group (P> 0.05); 5.GSH change, heart, lung, liver, skeletal muscle, serum GSH content of YTF, YHF group than the YAF group is high, and the lung YAF groups in YHF group there was significant difference (P <0.05), serum YTF group compared with the YAF group significant difference (P <0.05); 6.AGEs change, heart, lung, liver, skeletal muscle serum AGEs levels YTF, YHF group were lower than the YAF, and the hearts of YHF group compared with the YAF group significant difference (P <0.05), the lung content of AGEs YTF group and YHF, YAF group are there was significant difference of low (P <0.05).Conclusions:1, different loads swimming (t = 45min, 90min) can regulate aging in rats non-enzymatic glycosylation role in maintaining the stability of antioxidant system to achieve the anti-aging effect;2 different loads swimming (t = 45min, 90min) can enhance the aging body's antioxidant capacity in rats;3 different loads Swimming (t = 45min, 90min) can enhance the body's aging rats to non-enzymatic glycosylation inhibition;4, the weight change only as a movement of animals observed anti-aging auxiliary indicators.