Study On The Effects And Mechanism Of Dachengqi Decoction And Rhein Treating Endotoxemia

the Da Cheng Qi Decoction (DCQTD) is the main prescription of curing for empirical Yang Ming organs in traditional Chinese medicine; it has been used for curing endotoxemia and has obvious effect in recent years. But up to now the mechanism of its action especially the relationship between clinical effects and chemical components of DCQD is still controversial, so its formulation reform and quality standards can't be fixed, then its clinical application and new drugs'research are subjected to certain restrictions.Objective:This text evaluates the treatment of DCQD and its different extracts on endotoxemia by the index of plasma endotoxin, immunohistochemistry, biochemical testing and histological, then explore the mechanisms and efficacy of parts of DCQD, and then provide the basis for exploring the material basis of efficacy and mechanism of action of DCQD.Methods:Divide 126 male KM mice into 7 groups randomly, groups are separately control group,model group, DCQD group, chloroform group, ethyl group, ethanol group and ethanol precipitation Group,18 KM mice each group. Mice of control group are abdominal injected NS(10mg/kg) sterile, the others are abdominal injected LPS (10mg/kg) the treatment groups are given remedy liquid (8.11g/kg·d) by Ig at 0.5h before and lh after attacking and 0.5h before collecting blood; the control group and model group are given NS at the same time.8 hours later, collect 0.5ml ventricular blood under sterile conditions and detect the plasma ET, collect parts of the liver to have the immunohistochemistry, and take the heart. Liver and kidney to have the histological. Then explore the treatment of DCQD and its different parts in Endtoxemia; at the same time, divide 54 male KM mice into 3 groups randomly, groups are control group, model group and rhein group,18 KM mice each group, the ways of Ig and modeling are the same as the above. And then observe the changes of endotoxin, inflammatory cytokines in liver tissue and liver tissue biopsy in mice of endtoxemia by modeling, to explore the protecting effect of rhein on mice of endtoxemia.Results:The effect of DCQD group and ethyl group are significantly better than other curing groups on improving the plasma endotoxin, TNF-α, IL-1β, IL-10, MDA and the pathological inflammatory activity of heart, liver and kidney.Conclusions:The DCQD and its ethyl acetate and rhein all have obvious effects on Endotoxemia, the mechanism may be the following:promote the recovery of gastrointestinal function, prevent the bacterial translocation, decrease the absorption of endotoxin; reduce the excessive inflammatory response, improve symptoms, stabilize the balance of the body's immune; scavenging oxygen free radicals, protect liver sinusoidal endothelial cells; and protect the heart, liver, kidney and other organ functions and so on.