| In allusion to the artificial Cordyceps sinensis of Yi Kang company in Hunan province, this topic mainly analyzed content and structure of the effective components (polysaccharide, nucleoside, amino acids) in different parts of artificial cordyceps, and study the protective function of polysaccharide from different parts of cultured Cordyceps on carbon tetrachloride(CCl4)-induced liver damage in mice. It expect for providing a reference for taking full advantage of artificial Cordyceps sinensis resources.Methods:(1) Polysaccharide in the fruiting bodies, the mycelium and the solid mediumwas extracted by water and separated by ethanol, which was degreased by petroleum ethe and decolored by activated carbon. The protein was excepted by sevag method. UV Spectrophotometer determinated the content of polysaccharide in different parts of the artificial cordyceps sinensis.(2) The overall polysaccharide was separated by chromatography on a DEAE column and Sephadex G-100 High Resolution column, Relative molecular weight of the polysaccharide was measured by HPLC-ELSD. (3) RP-HPLC analysed the content of cordycepin, adenosine and adenine in artificial Cordyceps, using UV-Vis detector.(4) Amino acid was detected by amino acid analyzer L-8800 with post-column derivation, which was derived by Ninhydrin reagent.(5) Studied the protective function of polysaccharide from different parts of cultured Cordyceps on carbon tetrachloride (CC14)-induced liver damage in mice. Established liver damage model of mouse. After druging for a week, determinate and compared the effective index of liver index, and the liver morphology was observed.Results:(1) The content (μg·g-1) of polysaccharide in the fruiting bodies, the mycelium and the solid medium of artificial Cordyceps was 10.40%,4.73%,1.86%, respectively.(2) The experimental results showed that the linear range was Mw between 5000 and 70000. Standard curve equation was gained as lgMw=-3.3198tR+21.453, r=0.9995. The average molecular weight of the fruiting bodies was 49597; that of the mycelium was 15002; that of the solid medium contained three kinds of polysaccharide, the molecular weight was 30521,5980,4453, respectively. This method was found to be sensitive and accurate for the measurement of polysaccharides in Cordyceps sinensis.(3) The content (μg·g-1) of adenine in the fruiting bodies, the mycelium and the solid medium of artificial Cordyceps was 197.29,144.05,70.08, respectively; that of adenosine 1302.52, 308.17,71.51, respectively; and that of cordycepin 1356.92,1 101.04,350.96, respectively.(4) The solid residues, the fruiting body and the mycelium of cultured Cordyceps all contain 17 kinds of common amino acids, the total amino acid content was 8.32%,21.68%,23.19%, respectively. What is more, they all contain eight kinds of essential amino acids. Essential amino acid contents was 3.74%,8.11%,8.88%, respectively.(5) In the sporophore, mycelium and solid medium polysaccharide groups, the increases of AST and ALT activities in serum and MDA in liver were inhibited obviously. The decreases of liver SOD were reversed actually. The lesions in liver were ameliorated obviously. Sporophore polysaccharide was better than mycelium polysaccharide on protective effect on acute chemical liver injury induced by CCl4 of mice, and solid mediumwas the third.Conclusion:(1) Artificial cordyceps sinensis mycelium and solid medium contain a certain amount effective component (polysaccharide, nucleoside, amino acids).(2) Artificial cordyceps sinensis sporophore, mycelium and solid medium showed protective effects on the acute chemical hepatic injury induced by CCl4 of mice, but three groups not seen significant difference. Divot and solid culture medium can been further exploited in resistance to liver damage. |
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