Studies On Artificial Domestication And Culture Of Cordyceps Sinensis And Its Efficacy Against Chronic Kidney Disease

In recent years, kidney diseases was the highest lever in the world, its treatment takes a longer timeand cost a lot but often defeats its own purpose. Now, it becomes one of the important problems in theworldwide. Based on the observation and statistics on the treatment of chronic renal diseases withCordyceps militaris been obtained by the experiment, the experiment data and microbial strain resourceshave been presented. It is useful for the research and development on the diagnosis.Cordyceps militaris Link belongis to Cordyceps, Clavicipitaceae, Hypocreales, Sordariomycetidae,Ascomycetes, Ascomycota, Fungi. It is belonging to the same genera, and has approximate chemicalingredient and medical efficacy with Cordyceps sinensis. C. militaris contains cordyceps polysaccharides,cordycepic acid, cordycepin, many micro elements and various amino acids, reduces the albuminuria, so itcan improve immunity, improve the blood flow of kidaey and its function.Three Cordyceps militaris strains obtained using domestication and filtration technic methods. Thestrain Cm-T2is high quality in cordyceps polysaccharides, cordycepin and selenium, so it has a very highmedicinal value. Rice culture medium is the best solid medium in the study of three media. Through aseries of tests, the optimized cultivation parameters of Cordyceps militaris were obtained as follows.Mycelial culture: keep in dark, dispense with airiness, humidity60~70%,15~20℃former3days,23~24℃later. Change color and speed up bud:20℃12h，15℃12h，need5℃stimulus, humidity70~80%,astigmatism12h, opportune airiness. Fructification administer:20~25℃, humidity70~80%early daysthen75~85%when fruiting body0.5cm, astigmatism14h, airiness, give small puncture when fruitingbody2~3cm.Thirty patients with chronic renal diseases divided it into two groups (A&B): Group A, theexperimental group, to take Cm-T2with routine medication; Group B, the control group, only using routinemedication. Two groups showed quantificational comparison of24h proteinuria excretion.In experimental Group A, there was a significant difference (Single factor variance analysis: a=0.01,F0.01(2,42)=5.18, F=172.13>5.18) before treatment, after treatment thirty days and sixty days. According Single factor variance analysis, significant differences have been showed before treatment andafter treatment thirty days (a=0.01，F0.01(1,28)=7.64, F=194.10>7.64), before treatment and aftertreatment sixty days (a=0.01，F0.01(1,28)=7.64, F=254.63>7.64), after treatment thirty days and aftertreatment sixty days (a=0.01，F0.01(1,28)=7.64, F=37.10>7.64). The results of24h proteinuriaexcretion are obvious. It showed that proteinuria decrease after thirty days and also after sixty days.In traditional therapy Group B, there was a significant difference (Single factor variance analysis: a=0.01，F0.01(2,42)=5.18，F=6.29>5.18) by before treatment, after treatment thirty days and sixty days.According Single factor variance analysis, significant differences have been showed before treatment andafter treatment thirty days(a=0.01，F0.01(1,28)=7.64, F=9.15>7.64), before treatment and aftertreatment sixty days(a=0.01，F0.01(1,28)=7.64, F=11.89>7.64). According Single factor varianceanalysis (a=0.01，F0.01(2,42)=5.18，F=0.14<7.64), no significant difference had been showed, itrevealed that proteinuria decrease slowly after thirty days to the second month.Used the data of Group A and Group B on the first month and the second month by treatment, singlefactor variance analysis shows a significant difference (a=0.01, F0.01(1,28)=7.64, F=12.47>7.64; F=37.96>7.64). This shows that in the fist month the therapy of Group A is better than Group B on reducing24hours urine protein, and the efficacy in the second month is more obvious than in the fist month.The result showed that domesticated strain Cm-T2has medicinal value on strikingly decreasingproteinuria. The present thesis will contribute to the new drugs research and experimental data on treatmentof chronic kidney disease, and contribute to the development of Cordyceps Tea and Cordyceps Capsules aswhile as improving health standards in society in future.