| Background and objectiveThe essential hypertension is one kind of chronic illness, that is based on the basis of interaction between polygenes hereditary and the environmental factor. It is also one independent hazard factor of cardiovascular diseases such as coronary heart disease, cerebrovascular diseases such as stroke and finally the end stage of kidney failure, which does harmful to people's health.It is the foundation of early diagnosis, prevention and treatment of essential hypertension that screening related gene's single nucleotide polymorphism of essential hypertension and investing the relationship of gene polymorphism and essential hypertension plus under pressure effect of antihypertension drugs.This paper includes two chapters. The first chapter is to investigate the relationship between endothelial nitric oxide syntheses gene (eNOS) G894T and endothelin-2 (ET-2) gene A985G polymorphisms and essential hypertension in elderly. The second chapter is to investigate the relation between under pressure effect of Felodipine (FLDP) and endothelial nitric oxide synthase gene G894T and endothelin-2 A985G polymorphism.MethodsChapter 1:A case-control study was performed to study the association between eNOS and ET-2 gene polymorphism and hypertension by gene chip technology. The genotype and allele frequency distribution were compared between groups.Chapter 2:After primary hypertension patients took Felodipine for six weeks, groups were divided by eNOS genotype and ET-2 genotype, blood pressure degression value of different genotypic combination patients were compared.ResultsChapter 1:l.The frequencies of GG, GT and TT genotypes at eNOS 894 were59.1%,34.3% and 6.5% in hypertensive subjects and 76.3%,19.9% and 3.8% in normotensive subjects (χ2=13.745, p<0.05). The frequencies of G,T alleles at eNOS 894 were 76.3%,23.7 %in hypertensive subjects and 86.3%,13.7% in normotensive subjects (χ2=13.204, p <0.05)2. The frequencies of AA, AG and GG genotypes at ET-2 985 were 1.7%,21.7% and 76.5% in hypertensive subjects and 7.0%,35.5% and 57.5% in normotensive subjects (χ2=19.358, p<0.05). The frequencies of A,G alleles at ET-2 985 were 12.6%,87.4% in hypertensive subjects and 24.7%,75.3% in normotensive subjects (χ2=20.452, p< 0.05)3.Logistic regression was used to assess interactions between sex,BMI,free blood glucose,serum lipid,eNOS G894T genotypes and ET-2 A985G genotypes and essencial hypertension (p<0.01).Chapter 2:1. The systolic pressure and diastolic pressure of all patients are both reduced after taking felodipine (t=39.58, p<0.05; 32.14,p<0.05)2. the systolic pressure and diastolic pressure reduction values of TT genotypic patients were obviously higher than that of GT, GG genotypic patients (F=3.192, p<0.05; 3.289,p<0.05). The systolic pressure and diastolic pressure reduction values of ET-2 gene GG genotypic patients were obviously higher than that of AG, AA genotypic patients (F=14.135,p<0.05; 6.686,p<0.05).3. The systolic pressure of eNOS gene TT+ GT combined ET-2 gene GG+AG genotypic patients reduced as well, it had difference compared with that of other genotypic combination patients (F= 5.196; p<0.05).4. Binarry Logistic Regression results indicated eNOSgene TT genotype and ET-2 gene GG genotype was the chief influential factor of systolic pressure reduction in patients (OR=5.539,p<0.05; 15.673,p<0.05)Conclusions1. In summary, our data supporting sex,BMI,free blood glucose,serum lipid are independent risk factors for hypertension.2. Our data suggest that eNOS G894T variant and ET-2 A985G variant are major risk factors for essential hypertension in China.3. eNOS gene TT genotype and ET-2gene GG genotype are both associated with the reducing pressure effect of Felodipine; The subjects with the combination of eNOS TT and ET-2 GG genotype might have a better response of reducing pressure to Felodipine than that of the other genotypic combination patients. |
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