Astragaloside Aging Helf Cells Telomerase Activity, Cell Cycle Factors And Klotho Gene Regulatory Role In Experimental Research

Objective:To probe into the effects of astragaloside on the activity of SA-β-gal and telomerase,and the mRNA expression of cyclinD1,cyclinE,CDK4,p16,p21 and klotho,an anti-aging gene,on human embryonic lung fibroblast.Methods:We dividd HELF into four groups in this study,they are group of young,group of astragaloside,group of control and group of aging.In vitro human embryonic lung fibroblast,add the astragaloside when the cell grow up to 50 generations in test group.We observe the cell shape with the inverted Microscopy,and measured the cell viability by MTT method,the activity ofβ-Galactosidase by Dimiri's method,the activity of telomerase TRAP-PCR by Silver staining method,the mRNA expression of cyclinD1,cyclinE,CDK4,p16,p21 and klotho by RT-PCR method.Results:Compare with the youth group,the aging group cell viability and telomerase activity descent,β-galactosidase activity and the mRNA expression of cyclinD1,cyclinE,p16 and p21 goes up(p<0.05),the expression of klotho and CDK4 was fall down (p<0.05);compare with aging group,the activity ofβ-Galactosidase and the expressiones of cyclinD1,cyclinE,p16 and p21 were reduced,which effected by astragaloside.The cell viability,the activity of telomerase,the mRNA expression of CDK4,and klotho raise up.Conclusions:Astragaloside react the effect of anti-aging by enhanced to the activity of telomerase and mRNA expression of cyclinD1,cyclinE,CDK4,p16,p21 and klotho.