| Objective: In this study, using of classic hypoxic-ischemic brain damage(HIBD) animal model, Observate the changes of nerve cells,nerve fiber, Tau protein and phosphorylationTau protein before and after using of IGF-1.After using of IGF-1 in HIBD neonatal rats,discuss the relationship between the level ofTau protein,phosphorylation Tau protein and brain damage.Methods: 7-day-old Wistar rats are randomly divided into sham operation group model group and medicine group.Give the same dose PBS(sham operation group and model group)and IGF-1(medicine group)through peritoneal injection ,collect specimen after using medicine 24 houres.we observe the pathological change with hematoxylin and eosin staining and silver staining,using immunohistochemine and Western Blotting detect the dynamic changes of microtubule-associated proteinTau and phosphorylated Tau protein in alba.Results:. (1) sham operation group neonate rats HE:the shape of nerve cell is normal, adumbration is clear, nucleolus and nuclear membrane are obviousl,the size has no change,and don,t see changes of damage;HIBD neonate rats HE :Capillary hemorrhage, karyopyknosis , nuclear fragmentation ,the model making is successful.(2) sham operation group silver staining :the staining distribution of nerve cell is uniformity, the distribution of dendrite and axon are along with their lines;model group silver staining :nerve fiber thickening,some nerve fiber are tangled, medicine group nerve fiber are slightly thickening,and have no evidently nerve fiber tangled.(3)Immunohistochemine and Western Blot detect Tau protein and phosphorylated Tau protein of medicine group is reducer than the model group ,but higher than the sham-operated group (p <0.05). the changes trends of Tau protein band and phosphorylated Tau protein band is consistent.Conclusion:IGF-1 periphery using has protection for HIBD neonate rats,inhibite Tau protein phosphorylation,this is maybe one of the cell mechanism of IGF-1 neuroprotective effect. |
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