Fos And Jun Protein Bcl-2 And Bax In Protein Expression After Focal Cerebral Ischemia And Reperfusion Qi And Blood Activating Drugs On Its Expression,

Purpose:Programmed cell death(PCD)was one of ways that neurons died after cerebral ischemia and reperfusion. PCD took very important effect on neurons' death in delayed neuronal death (DND). It had been reported that expression of immediate-early genes(IEGS) and apoptosis-related genes were closely associated with PCD. In theories of Traditional Chinese Medicine (TCM), the mechanism of ischemic stroke was deficiency of Qi and blood stasis. Prescription of reinforcing Qi and activating blood had been using in clinic for a long time. It had positive effects significantly, but the mechanism hadn't been known. The mechanism of drugs of reinforcing Qi and activating blood to protect neurons after ischemic stroke was investigated by means of effects of drugs of reinforcing Qi and activating blood on the expression of Fos protein > Jun protein > Bcl-2 protein and Bax protein.Methods:String inserting method through external carotid artery was used to make the model of focal cerebral ischemia and reperfusion. ㏑adix salviea miltiorrhizae(RSM)> astragus and prescript of RSM and astragus were injected through abdominal cavity before ischemia in rats. The immunohistochemistry and MIPS were used to measure number and mean grey level of positive cells of Fosprotein , Jun protein.2 Ligustrazine (LZ), astragus and prescript of LZ and astragus were injected through abdominal cavity before ischemia. The immunohistochemistry and MIPS were used to measure number and mean grey level of positive cells of Bcl-2 protein, Bax protein in rats' cortex of ischemic sides. Results:1. The expression of Fos protein and Jun protein in model group increased significantly, compared with sham-operated group; RSM, astragus , RSM and astragus all could inhibit partly the expression of Fos protein , Jun protein after cerebral ischemia and reperfusion; Prescription of RSM and astragus had stronger inhibiting effects than RSM or astragus.2. The expression of Bcl-2 proteinN Bax protein in model group increased signicantly, compared with sham-operated group; LZ, astragus , LZ and astragus all could increase the expression of Bcl-2 protein and inhibit the expression of Bax protein at the same time; prescription of LZ and astragus had stronger effects than LZ or astragus.Conclusion:1. The expression of Fos protein , Jun protein Bcl-2 protein and Bax protein increases significantly after focal cerebral ischemia and reperfusion. It suggests that the exprssion of IEGS and apoptosis-related genes can be induced by cerebral ischmia and reperfusion. It is verified that IEGS and apoptosis-related genesplay important roles in PCD.2. The drug of activating blood , the drug of reinforcing Qi and the prescript of activating blood and reinforcing Qi all could partly inhibit expression of Fos protein or Jun protein to protect neurons after cerebral ischemia and reperfusion. The drug of activating blood, the drug of reinforcing Qi and the prescript of activating blood and reinforcing Qi all could increase the expression of Bcl-2 protein and decrease the expression of Bax protein to inhibit apoptosis and atteunate brain injury after cerebral ischemia and reperfusion.3. The prescript of reinforcing Qi and activating blood has stronger effects on the expression of IEGS proteins and apoptosis related proteins than either drug of reinforcing Qi or drug of activating blood. It is verified that there is synergism action between the drug of activating blood and the drug of reinforcing Qi. It may be one of mechanisms of reinforcing Qi and activating blood circulation therapy to treat ischemic stroke in clinic.