Medicine Fumigation Treatment Of Rheumatoid Arthritis Clinical And Animal Experimental Research

Objective:To evaluate the efficacy of the Chinese herb fumigation and steaming therapy (CHFS) for RA and AA rats,and detect the expressions of nuclear factorκB in the synovialis,in order to explore the anti-inflammatory mechanism of CHFS.Methods:1.Taking RA as the research object,divided into group and non-fumigation group,the study uses ACR20,50,70 and VAS standard to evaluate the clinical efficacy, and simultaneous monitoring of inflammatory indices and blood routine.2.Taking RA,OA and non-arthritis patients for joint replacement as the research object,Using RT-PCR method to detect the expression of NF-κBmRNA in synovial;Four cases of RA in which patients were given the comprehensive treatment for CHFS + MTX + Meloxicam + Xiangdan Injection,compared the effect of changes in indicators and the expression of NF-κBmRNA in synovial pre and post treatment.3.Project six rat groups of bland control, normal group,model group,water fumigation group,CHFS group,MTX group,MTX + CHFS group,study on the AA:Testing the expression of NF-κBmRNA in synovial of AA and to observe the number of joint swelling and changes in AI pre and post treatment.Results:1.After 20 days treatment,the indicators of the efficacy of CHFS group(ACR20, 50,70,VAS standard,PGA,DGA,the number of joint tenderness and swelling,HAQ, efficacy indicators of Traditional Chinese Medicine) and blood(Hb,PLA) improved,and the acute inflammatory indicators(RF,ESR,CRP) decreased,the results were significant compared with significant non-fumigation group patients(P＜0.05,P＜0.01 );2.The expression of NF-κBmRNA in RA Synovium is higher than the expression of OA and normal group,the results of the differences were significant(P＜0.01);RA,OA patients with rheumatic fever certificate-based expression of NF-κBmRNA higher than the cold wind Syndromes(P＜0.05);In CHFS plus drug treatment,as RA indicators of clinical efficacy and inflammatory changes of the synovial expression of NF-κBmRNA also fall.3). After 20 days treatment,the NF-κBmRNA expression of CHFS group was significantly lower than that model,the difference was significant(P＜0.05);The CHFS group compared with the MTX group and MTX + fumigation group,the difference was not significant (P＞0.05).Conclusion:1.The CHFS treatment has a good anti-inflammatory and analgesic effect of swelling;2.There has a better clinical efficacy for CHFS plus drug treatment,and its mechanism may through inhibiting the activity of NF-κB to play anti-inflammatory effects; 3.NF-κB has a high expression both in the synovial of RA patients and AA rat,which play an important role in pathogenesis;4.The NF-κB expression in RA synovium is significantly higher than that of OA and non-arthritis patients of joint replacement.5. Rheumatic fever type of RA,OA patients with NF-κB expression was significantly higher than the wind cold type,suggesting NF-κB will play an important role in the pathogenesis of inflammation.