Power For The Internal Capsule, Electrical Damage The Spasm Rat Model Of Central Monoamine Neurotransmitter Content Of The Research

Objective To Study the effect of electro-acupuncture on monoamine neurotransmitter of cerebral cortex and caudex dorsalis in spasticity model rats by electrolytic damage of internal capsule, and to explore the mechanism of action of monoamine neurotransmitter and to provide experimental foundation to for acupuncture therapy of spasticity by brain injured on clinic.Methods Establishd electrolytic damage of internal capsule model, then randomly individed SD rats into Blank group, Sham operation group, Electro-acupuncture DU Meridian Group, Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group. Electro-acupuncture DU Meridian Group rats were treated with acupuncture on " Da zhui(DU14) and Ming men (DU4)". Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group rats were treated with acupuncture on " Qu chi(LI11) and Yang lingquan (GB34)".The treatment was once daily and 30 minutes everytime, lasting for 3 days by 100 Hz continuous wave to shake limbs lightly. Observing the influence of electric acupuncture on muscular tension of the spasmodic model rats by BL-420e 4-channels electrophysiolograph.Fluorospectrophotometry analysis technique were used to investigate the effect of electro-acupuncture on the contents of NA,DA and 5-HT in cerebral cortex and caudex dorsalis.Results 1. After electrolytic damage of internal capsule ,Comparing with Blank group and Sham operation group, the extend amplitude of injured upper arm of rats in Model group and three electric acupuncture groups were decreased obviously (P < 0.01);After being treated,Comparing with Model group, the extend amplitude of injured upper arm of rats in three electric acupuncture groups were increased obviously (P < 0.01). 2. Comparing with Blank group and Sham operation group,the contents of NA,DA and 5-HT in the cerebral cortex of Model group were decreased obviously (P < 0.05);After being treated, comparing with Model group, the contents of NA,DA and 5-HT in the cerebral cortex of three electric acupuncture groups were increased obviously (P < 0.05).3. Comparing with Blank group and Sham operation group , the contents of NA in the caudex dorsalis of Model group were decreased obviously (P < 0.05) ,After being treated, compared with Model group , the contents of NA in the caudex dorsalis of three electric acupuncture groups were increased obviously (P < 0.05); Compareing with Blank group and Sham operation group, the contents of DA in the caudex dorsalis of Model group were increased,After being treated, compared with Model group , the contents of DA in the caudex dorsalis of three electric acupuncture groups were decreased; but not obvious varieties (P>0.05);Comparing with Blank group and Sham operation group , the contents of 5-HT in the caudex dorsalis of Model group were decreased,After being treated, comparing with Model group, the contents of DA in the caudex dorsalis of three electric acupuncture groups were increased, but not obvious varieties (P>0.05).Conclusion 1. Spasticity could be resisted by electro-acupuncture. 2. The effect of resisting spasticity by electro-acupuncture may relate to accommodate the contents of NA, DA and 5-HT in cerebral cortex and caudex dorsalis. 3. Comparing Electro-acupuncture DUMeridian Group, Electro-acupuncture Injured Side Group and Electro-acupuncture Uninjured Side Group each other, we founded the extend amplitude of injured upper arm of rats in three electric acupuncture groups did not have obvious difference., after being treated and the contents of NA,DA and 5-HT incerebral cortex and caudex dorsalis.did not have obvious varieties.