Atractylodes Poria Decoction Regulation Of Spleen Deficiency In Rats Vip Effective Parts Screening Studies

Objective: Use different polar organic solvents to extract the baizhu fulingdecoction, and got corresponding substance, which feeded to the mouses that is suffer from splenic asthenia to find out the effective site.Methods: Use N-butyl alcohol and Acetic ether to extract respectively the baizhu fuling decoction, got N-butyl alcohol component and Acetic ether component. Use Alcohol Shen-Water Extraction to extract Poria polysaccharide and Atractylodes Poria polysaccharide. Elution with 60% ethanol with Atractylodes baizhu fuling decoction macroporous resin column, by 60% ethanol eluate. Use The bitter cold diarrhoea and hungry eat to establish spleen-deficiency disorders animal model.Each of substance as a drug to treat rats. By RIA determination of plasma in the intestine VIP find the effective parts.Result: VIP levels in plasma comparison, Compared with the model group,N-butyl alcohol group and Baizhu Fuling decoction can reduce the Plasma levels of VIP( P<0.05 ). And the Baizhu Fuling group is apparent( P<0.01). Acetic ether , water, Poria polysaccharide and Atractylodes Poria polysaccharide group can low the level of VIP in the Intestine, but has no statistical significant. Acetic ether , water, Poria polysaccharide and 60% ethanol eluate group can increase the level of VIP in the plasma. Acetic ether group and 60% ethanol eluate group are most obvious (P<0.01).Conclusion: It imply that N-butyl alcohol parts is of the similar function with Baizhu Fuling decoction , which can reduce the rats of spleen deficiency levels of VIP.