| Objective:Perimenopausal depression is the first time in the incidence of perimenopausal, anxiety,depression,lack of energy as the main symptoms of the disease,that are affective disorder.Performance as irritability,anxiety,depression,disturbed mind,memory decline,a lack of confidence,slow,serious indifference to the outside world,loss of emotional response,or even develop into a serious depression.In recent years agrowing number of scholars began to perimenopausal depression,neurotran smitters that the brain function of the significant role of regulation can significantly improve depression in patients with clinical symptoms.Gengniananyi has in the sex hormones,osteoporosis, signal to the relevant gene expression,apoptosis and achieved a breakthrough progress.The topics to be from the behavior and monoamine neurotransmitter in the brain to explore Gengniananyi side of perimenopausal depression rat model of the mechanism.Methods:The 40 experimental mice age to 11 to 15 months for the natural perimenopausal female SD rats as experimental subjects,the weight of 220～280 g,were divided into Gengniananyi groups,estrogen group,Prozac group,model groups,each with 10 and another 10 selected three to four-month-old youth for the youth group,the weight of 100～120 g,the treatment group were fed with Gengniananyi soup,Premarin and Prozac, the model group fed with contour of saline,youth groups not addressed.In the day 0,9 days and 19 days respectively from each group said in weight and determination of one percent sugar water consumption.0 days and 19 days,the use of open-box test assessment of the behavior change,the first 20 days of the decapitated rats were killed,quickly stripping from the whole brain and brain tissue removed after weighing the cerebellum,Add to precold acid alcohol solution homogenized,blending after centrifugal from supernatant,and then use the method of fluorescence in the brain of monoamine transmitters norepinephrine(NE),5-hydroxytryptamine(5-HT),dopamine (DA)of the content and recording relevant data.Results: 1.Weight gain changes:The first nine days compared changes in body weight growth:the first experimental model of group nine days changes in weight when growth was significantly lower than that of youth,two,the difference is very significant(p＜0.01);Gengniananyi group,Prozac group and Premarin of the first group of nine days changes in body weight increased significantly higher than the model group,compared with the model group,the difference is very significant (p＜0.01);Gengniananyi group and Prozac group between the two groups,the difference was not significant(p＞0.05);Gengniananyi group and Premarin group between the two groups,the difference was not significant(p＞0.05).Article 19 days of growth changes in body weight compared:the first experimental model group 19 days of growth changes in body weight was lower than that of youth,two,the difference is very significant(p＜0.01);Gengniananyi group,Prozac group and the Group of Premarin and the 19th day of body weight significantly higher than the model group,compared with the model group,the difference is very significant (p＜0.01);Gengniananyi,and Prozac group worried Solutions Group between the two groups,the difference was not significant(p＞0.05);Gengniananyi group and Premarin group between the two groups,the difference was significant(p＜0.05).2.1 percent sugar water consumption changes:The first nine days of one percent sug- ar water consumption in comparison:model rats in the first nine days of one percent sugar water consumption(ml/100g body weight)was significantly lower than the youth group Rat,the two groups,the difference is very significant(p＜0.01);Gengniananyi, Premarin group rats in the first nine days of modeling one percent sugar water consumption higher than the model group,and model group,the difference was significant (p＜0.05),Prozac group and the model group,the difference is very significant (p＜0.01);Gengniananyi and Prozac group,the two group to compare,there was no significant difference(p＞0.05);Gengniananyi group and Premarin group between the two groups,the difference was not significant(p＞0.05).Article 19 days of one percent of water consumption of sugar in comparison:model rats in the first 19 days of modeling one percent sugar water consumption(ml/100g body weight)was significantly lower than young rats,the two groups,the difference There was a significant(p＜0.01); Gengniananyi group,Prozac group and Premarin group of rats in the first 19 days of modeling one percent sugar water consumption higher than the model group,with the model group,The difference was significant(p＜0.05);Gengniananyi and Prozac group, between the two groups,the difference was not significant(p＞0.05);Gengniananyi group and Premarin group between the two groups,the difference was not significant (p＞0.05).3.Behavior change:0 day of the first group of the level of activities(through Georgia),and vertical activities(vertical frequency)and no significant difference.The first 19 days of Open-field experiment in the level of activities(through Georgia.) Comparison:the level of activity in model group(to wear the number grid)was significantly lower than young rats,the two groups,the difference is very significant (p＜0.01);Gengniananyi,Prozac group and Premarin group level of activity of rats (wearing grid number)were significantly higher than the model,compared with the model group,the difference is very significant(p＜0.01);Gengniananyi group,Prozac group,between the two groups,the difference was not significant(p＞0.05); Gengniananyi group and Premarin group between the two groups,the difference was significant Significant(p＜0.05).The first 19 days of Open-field experiment in vertical activities(vertical frequency)comparison:model rats vertical activities(vertical number)was significantly lower than young rats,the two groups,the difference is very significant(p＜0.01);Gengniananyi group,Prozac group and Premarin group rats vertical activities(vertical number)were significantly higher than the model, compared with the model group,the difference is very significant(p＜0.01); Gengniananyi group and Prozac group,between the two groups,the difference was significant(p＜0.05);Gengniananyi group and Premarin group between the two groups, the difference was significant(p＜0.05).4.Brain neurotransmitter content:rat brain neurotransmitter NE of comparison: model rat brain neurotransmitter NE was significantly lower than young rats,the two groups,the difference is very significant(p＜0.01);Gengniananyi and Prozac group,the rat brain neurotransmitter NE were significantly higher than the model, compared with the model group,the difference is very significant(p＜0.01).Premarin group,model group between the two groups,the difference was not significant (p＞0.05);Gengniananyi group,Prozac group between the two groups,the difference was not significant(p＞0.05);Gengniananyi group,Premarin group between the two groups,the difference is very significant(p＜0.01).Rat brain neurotransmitter 5-HT of comparison:model rat brain neurotransmitter 5-HT was significantly lower than young rats,the two groups,the difference is very significant(p＜0.01);more Anyi in the group,Prozac group and Premarin group rat brain neurotransmitter 5-HT were significantly higher than the model,compared with the model group,the difference is very significant(p＜0.01);Gengniananyi group,Prozac group,between the two groups, the difference was significant(p＜0.05);Gengniananyi group and Premarin group between the two groups,the difference is very significant(P＜0.01).Rat brain neurotransmitter DA of comparison:model rat brain neurotransmitter DA was significantly lower than young rats,the two groups,the difference is very significant (p＜0.01);Gengniananyi,Prozac group and the rat brain neurotransmitter DA were significantly higher than the model,compared with the model group,the difference is very significant(p＜0.01),Premarin group and the comparison between the two groups, The difference was significant(p＜0.05);Gengniananyi group,Prozac group between the two groups,the difference was not significant(p＞0.05);Gengniananyi side of the group and Premarin Group between the two groups,the difference was significant (p＜0.05).Conclusion:The experiment proves that Gengniananyi soup can significantly increased perimenopausal depression rats weight growth rate,open-box test(Open-field experiment), the level of activities(to wear the number grid),vertical activities(vertical number), significantly increased 1%Sugar water consumption but also of depression perimenopausal period model of rat brain neurotransmitters(DA,NE,5-HT)was signifycantly higher,thus prompted Gengniananyi side can effectively improve perimenopausal depression model large The rat depression,can effectively adjust perimenopausal depression model of the hypothalamus-pituitary-axis of ovarian function,neuroendocrine coordination network,specific mechanisms have yet to be studied further. 属性ä¸ç¬¦... |
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