Jiang Xin Tang,. Ling Gan Flavors Expression And Regulation Of Experimental Studies, Chronic Bronchitis And Airway Inflammation In Rats Media

ObjectiveLingganwuweijiangxin Decoction, a famous prescription in Synopsis of theGolden Chamber, has the unique curative effect to chronic bronchitis of coldfluid-retention type through the practice by archaic and latter-day authority ofTCM.This decoction which is only consisted of five Chinese medicinal herbs, hasaccurate and apt compatibility which makes the decoction form precise.Its bestcharacteristic is that it combines transpiring with constringing and treates by both theprincipal and the secondary aspects of disease. The practice by archaic and latter-dayauthority of TCM has proved that the decoction dose a good power and makes a safeand reliable condition.This experiment aimed at probing into adjust and control effectof Lingganwuweijiangxin Decoction to expression of bronchial inflammatorymediators of chronic bronchitis of cold fluid-retention type rat. It opens up a newdirection for preventing and curing chronic bronchitis of cold fluid-retention type, andprovides objective basis for developping the decoction as a phlegm-dissolving andcough-stopping one.MethodsAccording to the theory of "Body's catching cold and drinking cold water willhurt lung", and using the method of building model that is type of rabbit fromguang-ren Sun and Insufficiency of the Lung-yang from Wangpeng,on this basis, thisexperiment reveals probing into adjust and control effect of LingganwuweijiangxinDecoction to expression of bronchial inflammatory mediators of chronic bronchitis ofcold fluid-retention type rat. Specific methods are as follows:1 The research with Lingganwuweijiangxin Decoction influence that theappearance learns to the chronic rat bronchus of the bronchitis and the lung: Theoptical microscope descends an observation to pay rat bronchus and lung to organizethe change that the appearance learns slowly, and attach to explain by photograph. 2 Make use of RIA method to observe the effect of LingganwuweijiangxinDecoction on the peripheral blood of rats with chronic bronchitis cAMP, cGMP levelsof expression.3 Make use of Immunohistochemistry method to observe the effect ofLingganwuweijiangxin Decoction on chronic bronchitis rat lung tissue of IL-1expression levels.4 Make use of nitrate reduction method to observe the effect ofLingganwuweijiangxin Decoction on the peripheral blood of rats with chronicbronchitis NO expression levels.Result1 By optical microscopy Lingganwuweijiangxin Decoction and Chuanbei loquatsyrup treated with bronchial and lung tissues of the observation ratio, found thatLingganwuweijiangxin Decoction can improve inflammatory factor on rat bronchialand lung injury, and is able to necrotic organization for reconstruction, recoveryorganizational structure, high dose group in particular. But the latter is not clear.2 Lingganwuweijiangxin Decoction rats the peripheral blood cAMP, cGMP andcAMP levels close to the blank group (p＞0.05), compared with the model group,there was significant difference (p＜0.05).3 Lingganwuweijiangxin Decoction rats the bronchial tissue IL-1-positive rateswere lowered that are close to the blank group (p＞0.05), compared with the modelgroup,there was significant difference (p＜0.05).4 Lingganwuweijiangxin Decoction rats the peripheral blood NO content werelowered,compared with the model group, there was significant difference (p＜0.05).Conclusion1 By optical microscopy, found Lingganwuweijiangxin Decoction can improveinflammatory factor on rat bronchial and lung injury, and is able to necrotic tissue forreconstruction, rehabilitation organizational structure.2 Lingganwuweijiangxin Decoction can improve the blood of rats with chronic bronchitis cAMP and cGMP balance Resumption of cAMP and cGMP cell response tothe regulation.3 Lingganwuweijiangxin Decoction can reduce chronic bronchitis rat bronchial andlung tissue of IL-1-positive rate, thereby suppressing the airway inflammatory cellinfiltration, reducing airway hyper-responsiveness, ease inflammation of furtherdevelopment.4 Lingganwuweijiangxin Decoction can reduce blood NO levels of chronicbronchitis, thereby improving pulmonary artery vasomotor dynamic balance,pulmonary vascular reconstruction, the Department of vascular endothelial integrity.