Dietary Calcium And Mechanism Of The Prevention And Treatment Of Obesity In Rats

Partâ… Effect of dietary calcium on the obesity of SD rats fed with high-fat dietObjective: To investigate the effect of dietary calcium on the obesity of rats fed with high-fat diet. Methods: After ten days of free access to a standard diet, 30 SD rats were randomly divided into 3 groups with 10 rats each. The rats in group A were fed with standard diet(1.30% calcium), those in group B with high-fat and normal-calcium diet(1.57% calcium),and those in group C with high-fat and high-calcium diet(2.57% calcium). At the end of 6 weeks, the rats were sacrificed.The body weight and the wet weight of fat pad were weighted. The serum element calcium were detected by using atomic absorption spectrophotometric(AAS) method. The serum calcitrophic hormones PTH and 1, 25-(OH)₂D₃ were measured with ELISA method. And the level of FAS, VDR and UCP₂ mRNA in WAT(White Adipose Tissue) were measured with RT-PCR. And the levels of FAS protein expression were validated by using Westen Blotting method. Results:â‘ The body weight, the Lee's index and the wet weight of fat pad were higher in group B than those in group A or C(P＜0.05);â‘¡The level of serum element calcium in group C were higher than those in group B(P＜0.05). The levels of serum PTH and 1, 25-(OH)₂D₃ in group C were lower than those in group B(P＜0.05);â‘¢Both VDR and FAS mRNA expressions in group C were lower than those in group B(P＜0.05), UCP₂ mRNA expression in group C was higher than that in group B(P＜0.05);â‘£group C were showed lower protein expression of FAS than those of group B(P＜0.01). Conelusion: Via decreasing serum calcitrophic hormones, high-calcium diet may influence fat metabolism and calcium metabolism, which can result in reducing the body weight and fat pad of the rats fed with high-fat diet. Partâ…¡Study on effects in obese SD rats given by high-calcium dietObjective: To explore the effects in obese SD rats given by high-calcium diet. Methods: After induced by high-fat diet for 6 weeks, 20 obese SD rats were randomly divided into 2 groups: group A were fed with high-calcium diet(2.30% calcium), group B were fed with standard-calcium diet (1.30% calcium), respectively. Then 2 groups were fed for another 6 weeks. At the end of experiment, the rats were sacrificed. The body weight and the wet weight of fat pad were weighted. The serum element calcium were detected by using atomic absorption spectrophotometric(AAS) method. The serum calcitrophichormones PTH and 1, 25-(OH)₂D₃ were measured with ELISA method. And the levels of FAS, VDR and UCP₂ mRNA in WAT were measured with RT-PCR. And the levels of FAS protein expression were validated by using Westen Blotting method. Results:â‘ The body weight, the Lee's index and the wet weight of fat pad were lower in group A than those in group B (P＜0.05);â‘¡The level of serum element calcium in group A were higher than those in group B(P＜0.05). The levels of serum PTH and 1, 25-(OH)₂D₃ in group A were lower than those in group B(P＜0.05);â‘¢Both VDR and FAS mRNA expressions in group A were lower than those in group B(P＜0.05), while UCP₂ mRNA expression in group A was higher than that in group B(P＜0.05);â‘£group A were showed lower protein expression of FAS than those of group B(P＜0.05). Conclusion: Via decreasing serum calcitrophic hormones to inhibit expressions of VDR, FAS mRNA and enhance expression of UCP₂ mRNA, high-calcium diet may be helpful to attenuation of weight and fat gain in obese rats with the effects of inhibiting lipogenesis and increasing temperature.