Calcium Metabolism And Its Related Genes In The High-fat Diet-induced Obesity Susceptible To Differences In Obesity Resistant Rats

Objective: To establish high-fat diet induced obesity-prone and obesity-resistant rats, and explore the differences of partial physiologic indexes including weight, the wet weight of pad and the expressions of calcium metabolic regulative hormones as PTH and 1_α ,25-(OH)₂-D₃. Methods: (1) To make the model of high-fat diet induced obesity-prone and obesity-resistant rats. (2) To detect the element calcium in serum by using atomic absorption spectrophotometric (AAS) method; determine the indicators of TC, TG, HDL-C in serum by enzymic oxidation method. (3) The calcium metabolic regulative hormones as PTH, 1 _a,25-(OH)₂-D₃ were detected by ELISA technique. Results: (1) After the preparation for obese rats, the speed of body weight gain and the wet weight of fat pad in OP rats were significantly higher than those in OR rats (P<0.001). (2) The serum element calcium in OP and OR rats were lower than those in standard control group; and the indicators of TC,TG in OP group were higher than those in standard control group(P<0.05). (3) The serum PTH content in OP,OR and high-fat group were higher than those in standard control group(P<0.05), and the former three groups were all lower of serum 1_α ,25-(OH)₂-D₃ than those compared with standard control group(P<0.05). Conclusions: To make the model of high-fat diet induced obesity-prone and obesity-resistant rats is according with the biochemistry characteristic of high-fat diet in public. High-fat diet leads to the change of the serum element calcium and the level of...