Cytochemistry Of The Pterygopalatine Ganglion Of Rats With Allergic Rhinitis And Its Changes In The Cervical Sympathetic Trunk Blocking After

Objective: 1. To investigate the expression level of nerve growth factor(NGF) and neural nitric oxide synthase(nNOS) in pterygopalatineganglion of allergic rhinitis rats; 2. To address the distribution of theneurons which postganglionic fibers innervate the nasal mucosa; 3. Tostudy the expression level of NGF and nNOS in PPG of allergic rhinitisrats following sympathectomy.Methods: 1. Using NGF and nNOS immunofluorescence stainingmethods respectively, the expression level of NGF and nNOS wasdetected in pterygopalatine ganglion of the allergic rhinitis rats; 2. Thedistribution of the neurons which postganglionic fibers innervate thenasal mucosa was mapped by the fluorogold(FG) and horseradishperoxidase(HRP) retrograde tracing respectively; 3. The NGF positiveneurons and the nNOS positive neurons were observed by the NGF andnNOS immunofluorescence in PPG of the allergic rhinitis rats followingsympathectomy.Results: 1. The modified model was good by the change of behavior andsymptom and the nasal mucosa stained by HE method; 2. The number oflabeled NGF-positive neurons and nNOS-positive neurons was increasedsignificantly (P＜0.01) in pterygopalatine ganglion of the allergic rhinitisrats; 3. The neurons which postganglionic fibers innervate the nasalmucosa were scattered in PPG, and the distribution of these neurons was no significant difference between FG and HRP retrograde tracingrespectively; 5. The decreased number of NGF positive neurons andnNOS positive neurons was detected in PPG of allergic rhinitis ratsfollowing sympathectomy.Conclusions: 1. NGF and nNOS were probably involved in thepathologic mechanisms of the allergic rhinitis by the nerve regulation; 2.parasympathetic fibers innervating the nasal mucosa also played animportant role in the pathologic mechanism; 3. The synthesis of NGF andnNOS in the parasympathetic neurons of PPG were regulated bysympathetic nerves, which was vital to the pathologic mechanisms ofallergic rhinitis.