Three Grass Buck Soup Antihypertensive Effect And Mechanism Of Experimental Research

There are three parts in this thsis literature summarize ,theory research and empirical study.1. Literature summaryThis thesis summarizes the study overview of Chinese medicine about treat hypertensive disease,and summarize the modern research progress about differentiation of symptoms and signs for classification of syndrome.2. Theory researchCombining past dynasties specialist discuss together with modern medicine study overview, we treat hypertensive disease from hepatic fire.3. Empirical study3.1 ObjectiveStudy san cao depressurization decoction (SC) how to treat hypertensive, observe its seasoning and dose-effect relationship, investigate the mechanism of action of depressurization, investigate the depressurization of SC and find the relationship with endothelin,nitrogen monoxidum,atrial natriuretic peptide and renin-angiotensin system.3.2MethodsWe adopt SHR and RHR two hypertension rat model, with the purpose to observe the depressurization of SC Master single administer test, investigate its seasoning and dose-effect relationship,observe the depressurization effect of long-term administration ,and investigate the heart rate,body weight and general behavioral.We deploy radio-immunity to observe the effect of SC on endothelin,atrial natriuretic peptide and renin-angiotensin system. We deploy nitrate reductase to observe the effect of SC on nitrogen monoxidum.3.3 Resuls3.3.1 Observe SC's seasoning and dose-effect relationship After drenching different dose SC on SHR,RHR rats, we found visible depressurization effect.Administration 2 hours, there is depressurization; 4 hours, to the peak amplitude;6 hours, it still manifest depressurization effect;8 hours , rebound to near pretherapeutic level. SC low dose didn't have obvious depressurization on SHR. SC midst dose and large dose have obvious depressurization on SHR. large dose have the best depressurization effect .therre are manifest dose-effect relationship. However,SC low dose have obvious depressurization on RHR rat, midst dose have the best depressurization effect, large dose have lower effect than midst dose.3.3.2 Observe SC long-term administration's effects on SHR,RHR rats'blood pressure,heart rate and general behavioral.SC long-term administration obviously relieve hypertension's nonage buildup tendency, inhibit hypertensive disease's further development. Comparing with synchronization model group, depressurization amplitude reach 15mmHg,with the demonstration that SC have corking depressurization effect.SC can decrease the irritability degree, diminish fight, clear urine, keep Sed willy-nilly of SHR rats. SC acan turn pneuma fine, add appetite, increase activity and improve color pattern and anischuria of RHR rats.SC didn't have evidence effect on SHR rats'body weight, but can increase RHR rats'body weight.3.3.3 Find The effect on endothelin,nitrogen monoxidum,atrial natriuretic peptide and renin-angiotensin system of SC .SC could obviously cut down SHR,RHR two rat model's endothelin density, step up nitrogen monoxidum density of blood plasma to regulate the two sides'balance. It may be the important mechanism of depressurization.Comparing to model group, ANP level step up predominantly arfter SC successive administration(P<0.01).It demonstrate that the mechanism of SC depressurization have correlation with how to regulate blood plasma ANP level of SHR rats. The RHR model group and the traditional Chinese medicine groop's blood plasma ANP level elevates obviously, there are not significant deviation between the two groups (P> 0.05).SHR rats normal group, model group and SC group in PRA content have not remarkable difference. The SC group and the model group Ang II content have not obvious difference, the function of prompting SC to fall to the SHR rats pressure is by no means through renin-angiotensin system adjustment. SC may obviously reduce RHR rats blood plasma PRA, the AngII content, prompts SC to fall to the RHR rats presses the function possibly is through to the renin-angiotensin system adjustment, thus achieved the goal to lower the blood pressure.3.4 Conclusions3.4.1SC single time administer has very well depressurization effect , and it have obvious seasoning and dose-effect relationship3.4.2 SC successive administration have very well depressurization effect , it may obviously alleviate SHR, RHR two kind of hypertension models rats in the hypertension early time blood pressure growth tendency, suppresses the hypertension course of the further development.3.4.3 SC not only reduced the blood pressure but also improve the symptom which appeared, it has manifested the Chinese medicine whole adjustment feature. 3.4.4 SC while falls blood pressure can obviously reduce SHR, RHR two kind of hypertension rats model's blood plasma ET density, elevates the blood plasma NO density, the adjustment two balance, this possibly is its the important mechanism of falls pressure function.3.4.5 SC can elevate SHR rats blood plasma ANP density, prompts SC to fall presure the function mechanism with to adjust SHR rats blood plasma ANP level, but SC adjustment is not certainly obvious to RHR the rats blood plasma ANP.3.4.6 SHR rats SC group and model group PRA, AngII content not obvious difference, prompts SC to fall pressure the function mechanism by no means source from to the SHR big mouse renin-angiotensin system adjustment .3.4.7 RHR rats SC group blood plasma PRA, the AngII content compares the model group remarkably to reduce, prompts SC to fall pressure the function mechanism with to adjust the RHR big mouse renin-angiotensin system adjustment to concern.3.4.8 SC falls pressure the action mechanism is various, SC falls pressure the function is, in view of the different model which completes through multi-targets has selectively.