Electric Rat Model Of Parkinson's Disease Substantia Nigra Dopaminergic Neurons Protective Mechanism

Objective: Parkinson's disease (PD) , a parapymmid progressive neurodegenerative diseases in centre nerve system , always invades middle-ages and older people and cause their disables. PD's mainly pathology change maintains the death of most of the dopamine neurons which project from tight zone of substantial nigra of midbrain to striatum. PD's mainly symptoms include stilly shacking, bradykinesia, rigidity muscles and abnormal posture and pace. Nowadays, western medicines could not prevent the progress of PD well, but bring about a lot of side effects at the meantime. Operation and gene therapy could not treat the PD well, either. At present, acupuncture as a peculiar, safety, and convenient way has been applied in the treatment of PD and has good effection. The text will study the therapeutical effect and mechanism of the electroacupuncture(EA) on Parkinson's disease (PD), which will offer experimental basis for applying EA in clinic praction of the acupuncture -moxibustion.Methods: 85 Wistar rats were allocated into four groups by random: PD group, EA group, sham-operation group and normal control group. PD model was established by microinjecting 6-hydroxy-dopamine into mesencephalic substantia nigra in the right brain. The same amount of normal saline was injected into the rats of control group. After 4 weeks ,Apomorphine was injected into the rats abdominal cavity to induce rotation . The test were repeated every 7 days .Rats who had constant left rotation with a speed faster than 7r/min were selected as successful PD models.41 successful PD model rats were selected for treatment and randomly divided into two groups, model group (n=20) and electroacupuncture group (n=21). Acupoints of Fengfu and Taichong in EA group were punctured once a day for 28 days. The results were analysed by F-test and q-test.1. Observation on behaviour: 0.5 mg/kg of apomorphine was injected into abdominal cavity to induce left rotation. The number of circles rotated within 30 minutes after injection of apomorphine were recorded, once a week for 4 weeks2. positive tyrosine hydroxylase (TH) neurons content in substantia nigra: After 6% chlorahydrate ( CH ) was injected to anesthetize the rats , the substantia nigra was taken out and maked into specimen in 6-8μm every piece. The immune constitution chemical method was adopted to measure the positive TH neurons , whose cell plasm should be dyed into tan.3. positive glial cell line-derived neurotrophic factor(GDNF) and...