Icam-1 And Other Cytokines Change In The State Of Rat Intestinal Ischemia-reperfusion And Its Effect On Intestinal Barrier Function

Objective To found out the mechanism of impaired intestinal mucosal barrier by studying the influence of cell factors and the relationship between them. Methods 60 healthy Wistar rats were randomized equally to study group, control group and normal group. For the well anesthetized study rats, exposing superior mesenteric artery (SMA), clamping the initiation of SMA and unclamp ing it one hour later were carried by routine. The control group were the same except for not clamping the SMA. 6 hours later, 1cm intestinal canal 15cm above the ileocecal junction were extracted and studied immunohistochemically, including the expression of ICAM-1, MMP-7, IL-6 and TGF- β 3. In addition, the semi-quantitative analysis by V1DAS image analysis system were carried either.Results In the study group, attenuated enteric wall, edema epithelium, short and thin intestinal villi and big space between them were obviously under the microscope. The neutrophil and lymphocyte soaked a lot in the experiment group. Immunohistochemical dyeing showed that the expression of ICAM-1, MMP-7, IL-6 and TGF- β 3 in the experiment group were higher comparing with that in control group(both P<0. 05). No difference was found between the control group and the normal group.Conclusion High expression of ICAM-1 and IL-6 lead to more expression of MMP-7 under stress. The high expression of TGF-β3 can not protect the intestinal mucosal barrier from inflammation. As a result, the intestinal mucosal barrier was destroyed and MODS came into being finally.