| ObjectiveChronic fatigue syndrome(CFS) is a disease that has been newly discovered by modern medicine and due to the social developments the incidence of CFS is on increase every year. So the importance of the research about CFS is increasing in every country through the world. But the etiology and the development mechanism of CFS is not very clear so far and as a result there have been no effective medical treatment method at the moment. From the view point of Chinese medical theory and clinical experience, the functional disturbance of the spleen is regarded as the inducing mechanism of CFS. Immune function abnormalities of were observed in a larger number of CFS patients. It was substantiated through clinical observation and a large number of experimental researches that CFS is closely related with the cell factors of immune system and that acupuncture treatment can control and reinforce the immune function of the body. In this research acupuncture treatments were given to the spleen deficiency type of CFS model mice. And the influences of acupuncture treatment to the related indices including cell factors were observed. The aim of this experiment was to confirm that acupuncture treatment can control the immune system of the body and there by eventually open the new area of Chinese medicine and clinical research regarding CFS.MethodsWister rats (male, weight 160-180g) were randomly distributed to control , model and electronic acupuncture groups respectively. Model and EA groups were given irregular feeding and cold water swimming as complex stress factors to make spleen deficiency model, and EA group received electro -acupuncture treatment at the same time.Electro-acupuncture(EA) group was punctured in Baihui(DU20), Zusanli(ST36), Sanyinjiao(SP6) for 21 days. Evaluation of the model was processed according to the behavior changes of the rats. Examining the content of the monoamine neurotransmitters in different brain tissues of the rats were measured by electrochemical and high pressure liquid chromatography methods. Examining the content of the IL-1β, IL-2 in serum were measured by enzyme linked immunosorbent assayResults1.Physical fatigue and hysteric behavior (mental fatigue) were observed from CSF model rats. They reflect that the rat model induced by complex stressors was basically succeful in simulating the pathogenesis and clinical manifestations of CFS. EA has certain control effects to the physical and emotional conditions of the CFS model rats.2.Disorder of monoamine neurotransmitters in central nervous system such as NE, DA decrease in the cerebral cortex, 5-HIAA in the hypothalamus were detected in the CFS model rats and EA group. EA could regulate the CFS model rat's disorder of monoamine neurotransmitters in central nervous system and selectively increase the level of the monoamine neurotransmitters in different parts of the brain.3.Metabolic disorder of cell factors in the serum were observed in CFS model group; levels of IL-16, IL-2 were increased which decreased in the EA group.Conclusions1.Stress, over consumption of physical strength, mental overstrain etc. are closely related factors which induce CFS. Spleen deficiency model made by irregular food feeding and cold water swimming as complex stress factors is a successful copy of the spleen deficiency type CFS as confirmed by observation of the clinical symptoms.2.Electro-acupuncture (EA) regulated the model rat's disorder of monoamine neurotransmitters in central nervous system, such as NE, DA, 5-HT and showed tendency of decrease in contents of serum IL-16, IL-2 to the normal status which increased in the model rats.3.Electro-acupuncture (EA) could markedly regulate the physical fatigue and the mental fatigue of the CFS model mice. |
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