Tumor Cell Chromosome Centromeric Dots (cd) Studies Of The Variants

OBJECTIVE: Chromosome centromeric dots are a pair of spherical structure that can be painted by special staining methods, which lies in the centromere of two chromatid's outer space. To study on staining methods of chromosome centromeric dots, to analyse and evaluate effect of two staining methods.METHODS: Giemsa method and Simultaneous silver staining technique of both NOR and Cd are used to stain metaphase chromosome specimen of human outer circular blood respectively.RESULTS: (1) Giemsa method is operated conveniently, but its stability is not good, structural definition of Cd is bad. (2) Simultaneous silver staining technique of both NOR and Cd is operated easily, its stability is good, structural definition of Cd band is good.CONCLUSION: Simultaneous silver staining technique of both NOR and Cd is operated easily, its stability is good, structural definition of Cd band is good. Its required reagent are cheap, it can be observed with a common optical microscope, thus it can be done in general laboratory Condition. Its specimen can be preserved long, Simultaneous silver staining technique of both NOR and Cd has a biggish applicable value practically.