Metastasis Suppressor Gene Nm23-h <sub> 1 </ Sub> Expression In Laryngeal And Analysis

[ABSTRACT] BACKGROUND The nm23 - H1 gene encodes a 17-kilodalton cytoplasmic and nuclear protein that has been shown to be reduced in a number of human carcinomas including breast, colorectal, lung, prostatic carcinomas. This study examines the immunohistochemical staining characteristics of the nm23 ?H1 protein in human laryngeal/hypopharyngeal carcinomas and their lymph nodes, vocal cord polyps, laiyngeal papilloma, and attempts to deteimine if there is any relationship between reduction of nm23 ?H1 protein immunohistochemistzy and the metastasis of lai~geal carcinomas. METHOD Routine streptavidin梑iotin immuno-histochemistty with a polyclonal antibody of nm23桯1 was employed to study the expression of the nm23桯1 protein in laryngeallhypopharyngeal carcinomas (N=32) and their lymph nodes (N2 1), vocal cord polyps (N=9), laiyngeal papilloma (N= 1). RESULTS All vocal cord polyps, laiyngeal papilloma and lymph nodes without metastasis did not show any immunostaining for the nm23? H1 gene product. However immunoreactivity was found in laiyngeal/hypopharyngeal carcinomas (N=32, 62.5%) and lymph nodes with metastasis (N=ll, 27.3%). The data were analyzed with the chi - squared (X 2) contigency test, PKO.05 and they were considered significant. CONCLUSIONS There is apparently reduced expression of the nm23 ?H1 gene in lymph nodes with metastasis compared with laiyngeal and hypopharyngeal carcinomas. It is possible that nm23 ?H1 gene plays a role in the metastasis of laryngeal and hypopharyngeal carcinomas. 2 ~X: 4$4~拁?~ nm23 - H1 1~...