Fluorocarbon Partial Liquid Ventilation In Respiratory Tract Histopathology Impact Of Inhalation Injury In Dogs

Objective: This experiment brought about histopatholofical alterations of respiratory tract factitiously by establishing the dog inhalation injury model. To investigate the histopatholofical alterations of animal model under the simple high frequency jet ventilation (HFJV) and HFJV plus PLy. We discussed the mechanism and feasibility of PLV treating on inhalation injury. Methods: 16 adult crossbred dogs were divided randomly into HFJV group and HFJV ?PLV group, these dogs were entercolia anesthetized with pentobarbital sodium, high frequency jet ventilator conducted HFJV to this dogs, the autonomic breathe were blocked by vein injecting scoline. Ejecting steam into respiratory tract made the inhalation injury models. The HFJV group were conducted HFJV with pure oxygen after2 hours, the HFJV+PLV group were conducted HFJV 盤LV. Both of the groups were killed after 6 hours, we observed the histopathological alterations of trachea, bronchus 4 and lung under the optical microscope and electron microscope. Results: (1) Under the optical microscope, the HFJV group had bronchial mucous layer defluxion, submucous congestion and edema, neutrophile granulocyte infiltration, lung edema, hemorrhage and nertrophile granulocyte infiltration. Under the electron microscope, this group had bronchial epithelial cells and submucous destruction, lung hemorrhage, endothelial cells destruction, alveolar Cells swell. (2) The pathological process of the HFJV盤LV grope had been less than the HFJV group obviously. Under the optical microscope, the HFJV+PLV group had partial or no mucous layer defluxion, less submucous congestion and edema, no nertrophile granulocyte infiltration basically, less lung hemorrhage and edema. Under the electron microscope, this group had less destruction of bronchial epithelial cells, basically integrate submucous cells, less lung hemorrhage, less endothelial cells lesion, less alveolar cells swell. (3) In the both groups, there had no significant difference between the left and right. Conclusion: The main pathological alterations of the inhalation injury are congestion, edema, hemorrhage and neutrophile granulocyte infiltration. PLV may play an important role in relieving the histopathological alterations of Inhalation injury such as congestion, edema and has anti-inflammatory function, it also can relieve the lesions of angioendothelial cells and alveolar cells, 5 suggesting some of the mechanisms in clinical application.