Long-shaped Nutmeg Processing Technology And The Role Of Research

Purpose:Nutmeg is embodied in The Chinese Pharmacopoeia .At present,there are two kinds of nutmeg in the market for sale, one is Mystica fragrans Houtt., the other is Mystica argentea Warb. In Indonesia,Mystica fragrans Houtt. is used as spices .however, Mystica argentea Warb is used as medicine.In China,the situation is just opposite,With the application of modern science and technology, our laboratory carried out comprehensive study, including comparative study of chemical composition and pharmacology of M. argentea Warb and M. fragrans Houtt.under the instruction of triditional chinese medicine theory.Material and method:1.Material: The material mentioned above was purchased in Bozhou Chinese Medincine Market,these material were identified as dry and ripe seeds of Mystica argentea Warb.The material mentioned above was purchased in Shenyang Chinese Medincine Market,these material were identified as dry and ripe seeds of Mystica fragrans Houtt.2.Method:(1)Comparative chemical composition study on the two kinds of nutmeg was carried out in the way of HPLC,TLC and UV,respectively, the two nutmeg, different processing methods, different commercially available, to compare the different medicinal parts, study the two Nutmeg composition differences, for the nutmeg into a further description of medicinal.(2)The experimental research on the two kinds of nutmeg and anti-inflammatory , In the anti-inflammatory experiments, were administered to give a long-shaped nutmeg raw materials, long-shaped nutmeg products, raw materials round nutmeg, mace products, high and low doses of round 9 days, by xylene-induced mouse ear swelling, Egg white induced edema model, determination of swelling and the inhibition rate,nutmeg and its processed products were compared on anti-inflammatory effect.Keeping healthy of spleen function of its processed products,Experiments in the spleen, the use of bitter cold diarrhea dismount and irregular diet of a rat model of spleen deficiency, successful model 4 weeks after oral administration were determined in rat skeletal muscle Na +-K⁺-ATPase and Ca²⁺ - Mg²⁺-ATPase activity was investigated two kinds of nutmeg and its processed products.Acute liver injury experimental research of the two kinds of nutmeg and its processed products,Acute liver injury in the protection experiment,each group 14 days after administration, with the peanut oil containing 0.12% CCl4 model of acute liver injury made, determination of serum ALT, AST, MDA, GST levels, before and after processing were compared nutmeg protect the liver.Result:1.Comparative study on the two kinds of nutmegFirst, the conditions of the thin screen, the best experimental conditions for thin layer of petroleum ether - hexane - chloroform - ethyl acetate (11:2:1.5:1);After comparison of the two kinds of nutmegs and its processing product in the method of HPLC and TLC,the ingredients are different in quantity, long-shaped nutmeg and its processed products of the active ingredients of methyl eugenol and iso-methyl eugenol content Nutmeg and its processed products in the round, and toxic and irritating ingredients myristicin ether content of less than sassafras round nutmeg and its processed products;Through long after baked bran, bran baked, fried with bran, bran after the bubble fried, steamed and baked bran layer of several processing methods and comparison of HPLC,After baked, after bran fried, steamed baked,in this three groups active ingredient of methyl eugenol and isomethyl eugenol content of differentiation is generally higher than the content of other groups,other content was lower than other groups;Obvious differences exists in their processing products,processing methods , parts ,and nutmegs for sale in markets.2.Pharmacodynamics preliminary comparative study of the two kinds of nutmegThe two kinds of nutmeg all have obvious anti-inflammatory effects In the anti-inflammatory experiments, both nutmeg and its processed products on the mouse ear swelling feet swelling in rats have a certain inhibitory effect, in which nutmeg best raw materials.In the spleen the experiment, through the skeletal muscle mitochondrial ATPase activity in comparison shows that: deficiency model group, Na⁺-K⁺-ATPase, Ca⁺-Mg⁺-ATPase activity of the minimum, the control group showed the highest activity, other groups of model group uniform is relatively higher, and the deficiency symptoms have eased, which Mystica fragrans Houtt.is the best group of nutmeg products.Obvious treatment effect can be get in curing acute liver injury.It has obvious effect in preventing liver injury. Acute liver injury in the protection of the experiment, the sample group and model group, AST levels were significantly reduced, ALT levels changed little in each group;Model group, the treatment group significantly decreased MDA content, including raw materials and long-shaped nutmeg nutmeg circle the best raw materials group,Compared with model group, treatment group could increase the GST, We believe that Mystica argentea Warb.is as effective as Mystica fragrans Houtt.and even better.Conclusion:This study continues to comparative study of both nutmeg,before and after processing,iso-methyl eugenol and methyl eugenol content and similar round nutmeg,however, low levels Myristicin,from the chemical composition,alternative nutmeg can be considered;Before and after processing of two kinds of Nutmeg,skeletal muscle mitochondria of the ATPase is different,two nutmeg processing both before and after,the protective effect of acute liver injury,Mystica argentea Warb. effect of the pharmacological effects of nutmeg or mace is better than Mystica fragrans Houtt.,from the pharmacological role,has once again proven the Mystica argentea Warb. can be used as alternative medicine or even use Mystica fragrans Houtt.According to comprehensive comparative study on quality,soaking technology, pharmacology of Mystica argentea Warb. and Mystica fragrans Houtt,Mystica argentea Warb. can be used as one of nutmegs.