ã€Objective】Study and establish the "Kidney Yang Deficiency" male sub-health rats model, and to observe the effects of Tiancanzhuangyang-san"on "Kidney Yang Deficiency" male sub-health rats model in body state, to observe the influence of immune function.ã€Methods】Using vitamin B4 (purine method) establish the "Kidney Yang Deficiency" male sub-health model of rats.According to random principle,All of the rats are divided into normal model group,sub-health model group,"Tiancanzhuangyangsan" group,"JingguishenqiPill"group,"GuhanYangshengjing"group. The different Groups use different drug, observe the weight each rats by using electronic balance, observe the rats' general situation and determination of rats thymus and spleen index, useing the ELISA test to detect immunoglobulin G, A and M level in serum, appraise the rats' Immune function condition by statistical analysis.ã€results】1.Using vitamin B4 intervention rats can make rats show the non-health state as "Kidney Yang Deficiency" symptoms, the MDA increasing and the SOD decreasing in serum, endocrine dysfunction (FSH decreasing,LH decreasing,T decreasinganf E2increasing.) and Tissue organ occur micronuclei degeneration changes, ang This kinds of change can be restored.it hints the method of establishing the "Kidney Yang Deficiency" male sub-health model of rats is successful.2.Tiancanzhuangyangsan can effectively improve the"Kidney Yang Deficiency" male sub-health model of rats' state.3.Tiancanzhuangyangsan Can increase the "Kidney Yang Deficiency" male sub-health model of rats' thymus and spleen weight, improve the thymus index and spleen index.4.Tiancanzhuangyangsan Can raise the"Kidney Yang Deficiency" male sub-health model of rats' immunoglobulinG, A and M level in serum.ã€Conclusion】1.Using vitamin B4 (purine method) may establish the"Kidney Yang Deficiency" male sub-health model of rats.2.Tiancanzhuangyangsan Can improve the"Kidney Yang Deficiency" male sub-health model of rats' immune function. |