Qi And Promoting Blood Circulation Drug Prevention And Treatment Of Rats With Heart Failure Cardiac Remodeling Of The Adjustment Mechanism

Objectives Our study to rat congestive heart failure models based on the Qixue theory as the guide, with microscopic image analysis, immunohistochemical techniques, such as observation Invigorating Qi and promoting blood circulation TCM drugs to early heart failure rat medicine in blood, myocardial tissue angiotensin II, norepinephrine, aldosterone, rennin, endothelin ET-1, tumor necrosis factor (TNF-a), interleukin-1 (IL-1), interleukin 6 (IL-6) and influence of rat cardiac function and morphological changes, reveal Invigorating Qi and promoting blood circulation TCM drugs drug prevention of early heart failure with targets and its mechanism. Link Explore Invigorating Qi and promoting blood circulation TCM drugs in heart failure rat medicine disease processes cardiac remodeling the prevention and control process, as well as Chinese native medicine for realizing the role of traditional Chinese medicine, link to early intervention to improve cardiac remodeling avoid then developed into heart failure treatment and provide basis for TCM related theory explains.Methods This experiment used method that intraperitoneal injection ISO for three consecutive days to establish the rat congestive heart failure model,respectively, were randomly divided into four groups for normal control group (hereinafter referred to as the control group), model group, qi and promoting blood circulation medicine group, and Tongxingluo group (abbreviation Tongxinluo group). Drugs after four weeks respectively, intervention echocardiography rat heart shape and detection function indexes, executed animals, take maximum crosscut parts left heart made pathology, the myocardial dyeing slice pathological form. Application Elisa according to kit manual regulation operation detects blood, myocardial tissue Ang angiotensinâ…¡(Angâ…¡), norepinephrine (NE), aldosterone (ALD), renin (PRA), endothelin ET-1, tumor necrosis factor (TNF alpha), interleukin-1 (IL-1 beta), interleukin 6 (IL-6) content. Results (1) Each rat echocardiography morphological index comparison model rats, LVSTd, LVPWTd, LVPWTs are significantly higher than other groups, significantly lower LVDd and LVDs, compared with controls, there is significant difference (P< 0.05). Qi and promoting blood circulation group, Tongxinluo group's LVPWTd LVSTd, LVPWTs reduced significantly, and LVDd, LVDs increased significantly, and, significantly compared model group, sexual difference (P< 0.05), and the curative effect of qi and promoting blood circulation better than tongxinluo group. (2) Model rats significantly lower than other groups to EF, compared with controls, there is significant difference (P< 0.05), qi and promoting blood circulation group, tongxinluo raised significantly, and group EF comparison model group was significant difference (P 0.05), and the curative effect of qi and promoting blood circulation better than tongxinluo group. (3)Light-microscopy rats pathological form compared myocardial tissue slices, model group appear myocardial cell hypertrophy, arranging disorderly, stroma cells infiltrating has inflammation, interstitial hyperplasia etc. Phenomenon, qi and promoting blood circulation group and grieved winding rats, myocardial morphology was significantly better than model group, and qi and promoting blood circulation medicine improved better than tongxinluo group. (4)Each rat left entricular mass index comparison results show:model rats than other heart body index increased significantly groups, compared with controls, there is significant difference (P< 0.05). Qi and promoting blood circulation group, tongxinluo group significantly lower heart body index group compared with model was significant difference (P< 0.05), and the curative effect of qi and promoting blood circulation better than tongxinluo group. (5) Each rat serum ANGII, en Renin, ET, ALD,-1 content is model rats, serum ANGII, en, ET-1 content increased significantly than other groups, compared with controls, there is significant difference (P< 0.05). Qi and promoting blood circulation group, tongxinluo rats, serum ANGII, en, ET-1 content with model reduced significantly, and group compared significant difference (P 0.05), and the curative effect of qi and promoting blood circulation better than tongxinluo group. (6) Each rat heart tissue of TNF-a, IL-, IL-1B six content is model rats, myocardial tissue TNF-a significantly higher than other groups, compared with controls, there is significant difference (P< 0.05). Qi and promoting blood circulation group, tongxinluo rats, myocardial tissue TNF-a reduced significantly, and group compared with model was significant difference (P< 0.05), and the curative effect of qi and promoting blood circulation better than Tongxinluo group.Conclusions Qi and promoting blood circulation medicine can inhibit heart failure rat early neural fluid system, reduce the transition of myocardial tissue activation of inflammatory cells factor leels, restrain and slow cardiac remodeling process and is do links such as muscle strength of effective prevention effect HF and alleviate the occurrence and development.