| Objective:This topic is to copy COPD rats model, through the smoked fatliquoring polysaccharide (LPS) the combination of endotracheal instillation method. It is guided by the nourishing qi and blood, chooses the traditional formulas. After the time box intervention of COPD rats model by Chinese medicine, to detect and analyze the pathological morphological changes of drug groups, and the difference of MMP-9/TIMP-1, TGF-betal, and VEGF in the lung tissue homogenate. Aim to further explore nourishing qi and blood and prescription of Yunnan Chinese medicine and the role of COPD airway remodeling pathological changes in different periods after the intervention. To clarify its mechanism, and to provide some experimental basis and theoretical basis for nourishing qi and blood and the prevention and treatment of COPD airway remodeling, and to better guide clinical prevention and treatment of the disease.Methods:1. GroupingNormal control group, model group, Chinese medicine intervention group (nourishing qi and blood, invigorate the circulation of high, medium and low dose group, nourishing qi and invigorating the circulation of medicine group of high, medium and low dose group, nourishing qi and blood high, middle, low dose group), each has a group at 0,4 weeks,8 weeks,12 weeks paragraphs. For positive drug group, each has a group at 4 weeks,8 weeks,12 weeks paragraphs.10 rats in each group, a total of 470 rats.2. Copy the COPD rats modelTo use smoke endotracheal drip into the smoked fatliquoring polysaccharide (LPS) joint method to copy the COPD rats model.The time of copying the COPD rats model is:the 0 week’s is 28 days,the 4 weeks’s is 56 days,the 8 weeks’s is 84 days,the 12 weeks’s is 112 days,and the model copy needs 28 days.3. The drug intervention According to experimental design points at different times for different groups.The time of starting delivery is:The 0 week’s begin with the second day,the 4 weeks’s begin with the 29th,the 8 weeks’s begin with 57th,the 12 weeks’s begin with 85th,the time of starting delivery in every period is 28 days.Drug intervention lavage 28 days in a row.4. Index detection(1) Observe the behavior change in experiental rats.(2) Produce the lung tissue pathological slices, viewer bronchioles in the lungs and perivascular inflammatory infiltration and the change of airway wall structure.(3) Enzyme-linked immunosorbent method, detection of COPD in the lung tissue of rats, VEGF, MMP-9/TIMP-1 TGF-betal change.Results:1. Each test index changes of rats at different times model group and normal control group:MMP-9/TIMP-1 ratio, model group is lower than the normal control group; VEGF, concentration of TGF-betal, model group was higher than normal control group, the differences were statistically significant (P<0.05).2. The change of various indexes after traditional Chinese medicine intervention in COPD rats.2.1 The change of MMP-9 after traditional Chinese medicine intervention in COPD rats.0 weeks,4 weeks,8 weeks period of Chinese medicine intervention group is higher than the model group, the difference was statistically significant (P<0.05).12 weeks period of Chinese medicine intervention group is higher than the model group, but YQYXHXG, YQYXG two groups compared with model group, there was no statistically significant difference (P>0.05).2.2 The change of TIMP-1 after traditional Chinese medicine intervention in COPD rats.0 weeks,8 weeks,12 weeks period of Chinese medicine intervention group is higher than the model group, the difference was statistically significant (P<0.05); 4 weeks period of Chinese medicine intervention group is higher than the model group, but YQHXG group compared with model group, there was no statistically significant difference (P>0.05), and the rest of the group compared with model group, the difference was statistically significant (P<0.05).2.3 The change of MMP-9/TIMP-1 after traditional Chinese medicine intervention in COPD rats.0 weeks period, period of 4 weeks,8 weeks,12 weeks period of the Chinese medicine intervention group compared with model group, Chinese medicine intervention group of MMP-9/TIMP-1 ratio with the decreased dose of Chinese medicine, the difference was statistically significant (P<0.05).2.4 The change of TGF-betal after traditional Chinese medicine intervention in COPD rats.8 weeks,12 weeks period of Chinese medicine intervention group is higher than the model group, the difference was statistically significant (P<0.05); 0 weeks period of Chinese medicine intervention group is higher than the model group, but YQYXHXG, YQYXHXZ, YQYXG, YQYXZ, YQHXG five group compared with model group, there was no statistically significant difference (P>0.05), and the rest of the group compared with model group, the difference was statistically significant (P<0.05); 4 weeks period of Chinese medicine intervention group is higher than the model group, but YQYXHXG, YQYXHXZ, YQYXG, YQHXG four groups compared with model group, there was no statistically significant difference (P>0.05), and the rest of the group compared with model group, the difference was statistically significant (P<0.05).2.5 The change of VEGF after traditional Chinese medicine intervention in COPD rats.0 weeks,8 weeks,12 weeks period of Chinese medicine intervention group is higher than the model group, the difference was statistically significant (P<0.05); 4 weeks period of Chinese medicine intervention group is higher than the model group, but YQYXHXG group compared with model group, there was no statistically significant difference (P>0.05), and the rest of the group compared with model group, the difference was statistically significant (P<0.05). Conclusion:1. Nourishing qi and blood, invigorating the circulation of medicine group, nourishing qi and blood group, nourishing qi and invigorating the circulation of medicine group can adjust the imbalance of MMP-9/TIMP-1, reduce the COPD airway remodeling in rat lung tissue homogenate TGF-betal, abnormal expression of VEGF, slow the development of COPD airway remodeling lesions.2. Nourishing qi and blood and the low dose of different group of prescription of Yunnan Chinese medicine of interference from the rat COPD model effect is more apparent, prompt that clinical equivalent dose can effectively relieve the pathological changes of COPD airway remodeling process; Through intervention of four periods for traditional Chinese medicine (TCM) after the intervention of COPD rats model, the indicators did not find the intervention effect of Chinese traditional medicine is an obvious difference in four periods.3. This study is through nourishing qi and blood and blood-activation therapy for clinical COPD provides the experimental basis and clinical thinking. |
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