Thymic Stromal Lymphopoietin Hormone Expression In A549 Cells And Drugs And The Effects

Part 1 The expression of thymic stromal lymphopoietin in A549 cellsObjective To investigate the expression of TSLP mRNA in A549 cells, and explore whether nuclear factorκB (NF-κB) is involved. Methods A549 cells were cultured with interleukin1β(IL-1β) and IL-4, or PDTC (NF-κB inhibitor). The expression of TSLP mRNA and protein were detected by RT-PCR and immunofluorescence assays.Results The expression of TSLP mRNA was significantly increased in A549 cells stimulated with IL-1βand IL-4 at 4h, and decreased at 10h, almost reduced to the baseline at 18h. PDTC greatly inhibited the level of TSLP mRNA. PDTC had inhibitory effects on the expression of TSLP mRNA. Conclusions Cytokines enhanced the inducible expression of TSLP in bronchial epithelial cells, and which was associated with NF-κB activation.Part 2 Effect of drugs on the expression of thymic stromal lymphopoietin in A549 cellsObjective To investigate the effect of the drugs (astragaloside IV and budesonide) on the expression of TSLP mRNA in A549 cells, and explore whether nuclear factorκB (NF-κB) is involved. Methods A549 cells were cultured with IL-1β, IL-4, or the drugs (astragaloside IV and budesonide). The effect of astragaloside IV or budesonide on the expression of TSLP mRNA were also quantified by RT-PCR. The cytoplasm protein expression of IκBαwas evaluated by western blot and the nuclear translocation of NF-κB P65 was detected by immunofluorescence assays respectively. Results The expression of TSLP mRNA and the nuclear translocation of NF-κB P65 were greatly abolished in A549 cells pretreated with budesonide. Astragaloside IV had little inhibitory effects on the expression of TSLP mRNA (P>0.05). However, Astragaloside IV suppressed the nuclear translocation of NF-κB P65, enhanced the protein level of IκBαin the cytoplasm.Conclusions: Inhibition of NF-κB activation and TSLP expression might be an essential therapeutic mechanism of budesonide for asthma therapy. Astragaloside IV had little inhibitory effects on the expression of TSLP mRNA, but astragaloside IV could suppress the activation of NF-κB.