| Recently, as a new analytical technique, resonance rayleigh scattering (RRS) method has attracted more attention and interests of analytical science for its remarkable characteristics of high sensitivity and simplicity. Gold nanoparticle, i.e. nanogold, show much potential applied value because of their special physical and chemical properties. At present, it has been widely applied to physics, chemistry, biology, optics, electronics, medication and clinic medicine. In this study, RRS spectra of the interaction of gold nanoparticle with L-cysteine, 6-Benzylaminopurine and cyclopropane were investigated. The spectral characteristics, affecting factors, properties of nano-gold conjuncts with the three kinds of matter and their analytical applications have been studied, and three RRS methods for their determination were developed, respectively. The merits are as following:In the BR (Britton-Robinson) buffer solution of pH 3.3, gold nanoparticle and L-cysteine react with each other to form a binded conjunct with bigger volume by the electrostatic attraction interaction,which make the diameters of the particles increased markedly, therefore, the RRS intensity can be enhanced greatly. The maximum RRS peak is at 389 nm and another peak at 362 nm. The RRS intensity is directly proportional to the concentration of L-cysteine in the range of 0.04～0.20 mg·L-1 (R=0.9990). Under the optimum reaction conditions, a detection limit of 6.5μg·L-1. L-cysteine at a signal-to noise ratio of 3 was obtained with the relative standard deviation (RSD) of 2.0%(0.08 mg·L-1, n=11). Meanwhile, the method showed a good selectivity over the co-existing amino acids. Therefore, a RRS method for the determination of L-cysteine with gold nanoparticle probe was developed.In BR buffer solution with pH 3.0, gold nanoparticles interacted with 6-Benzylaminopurine to form the aggregation of bigger volume by the electrostatic attraction and hydrophobic interaction. It made the diameters of the particles increased markedly, so that the RRS intensity was greatly enhanced and a new RRS spectrum was observed. There is a peak band between 300 nm and 420 nm. The maximum RRS peak is at 388 nm and there is another obvious scattering peak at 371 nm. The optimum conditions for the reaction, the effect of coexisting substances and the sensitivity have been studied. Using the optimum parameters, the relative RRS intensity is directly proportional to the concentration of 6-Benzylaminopurine in the range of 2.0～48.0μg·L-1 (R=0.9986). Base on this result, a new RRS method for the determination of 6-Benzylaminopurine was developed. The detection limit of 1.4 ng·L-1 6-Benzylaminopurine was obtained with the relative standard deviation (RSD) of 1.7% (40.0μug·L-1, n=11). This method was applied to determine the 6-Benzylaminopurine in MS culture medium sample with satisfactory results.In acidic BR buffer solution of pH 3.4, gold nanoparticles and ciprofloxacin reacted with each other to form a binding conjunct with bigger volume, which made the diameters of the particles incresened obviously, therefore, the RRS intensity was enhanced greatly and a new RRS spectrum was observed. The maximum RRS peak is at 361 nm and there are some other obvious scattering peaks are at 389 nm and 422 nm. In this study,361 nm was selected for the measurement of RRS intensity. The optimum conditions for the reactions, the RRS spectral characteristics and the sensitivity were researched and a RRS method for the determination of ciprofloxacin was developed. The relative RRS intensity is directly proportional to the concentration of ciprofloxacin in the range of 0.20～0.80 mg·L-1 (R=0.9993). The detection limit of 35.0μug·L-1 ciprofloxacin was obtained with the relative standard deviation (RSD) of is 2.0%(0.40 mg·L-1, n=11). The proposed method is successfully used to determine ciprofloxacin in tablet by RRS with gold nanoparticle probe. |
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