Studies On Chemical Constituents Of Hosta Plantaginea (lam.) Ascherson

Object To investigate the chemical constituents of Hosta plantaginea (Lam.) Ascherson, and provide a theoretical basis for quality control, clinical application, further development and utilization of this mongolia herb. Methods Solvent method was employed to extrac and partion. Antibacterial activities of all fractions were studied by Cylinder-plate method to screening the effective fraction of H. plantaginea. Chemical constituents were isolated and purified by solvent method and chromatographic method (silica gel,Sephadex LH-20,RP-18,macroporous resin HP-20,and so on).The structures of the compounds were elucidated on the basis of physiochemical identification and spectral analysis (MS,1H-NMR,13C-NMR,DEPT and 2D NMR). Results Fourteen compounds were isolated from the H. plantaginea and eleven of them were identified as eicosane acid (H1), (25R)-5α-spirostan-2α,3β-diol (gitogenin) (H2), hexadecanoic acid-2,3-dihydroxy propyl ester (H3), daucosterol (H4), kaempferol (H5), qercertin (H6), kaempferol-3-O-rutinoside (H7), kaempferol-7-O-β-D-glucoside (H8), gitogenin-3-O-{O-β-D-glucopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-galactopyranoside (H9), gitogenin-3-O-{O-β-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside} (H10), gitogenin3-O-{O-β-D-glucopyranosyl-(1→2)-O-[O-α-L-rhamnopyranosyl-(1→4)-β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-β-D-galac-topyranoside}(H11). Conclusions All of the Compounds were isolated from H. plantaginea for the first time. Compounds H1, H3, H4, H5,H6 and H8 were isolated from the genus Hosta for the first time.