| ObjectiveTo detect a lasting and stable method of generating rabbit model of sick sinus syndrome, and evaluate the effect of HCN2 transfected mesenchymal stem cells on the rabbit modelMethodsJapanese white rabbits were randomly divided into two groups, and the model of sick sinus syndrome was induced by catheter or formaldehyde chemical ablation。HCN2-GFP plasmid was extracted and transfected into mesenchymal stem cells cultured in vitro. Then the transfected cells were injected into right atrium of rabbit model to observe its effect on the model.ResultsComparing with formaldehyde chemical ablation, catheter ablation could depress the rest and inherent heart rate more obviously. There was no obvious difference between chemical ablation and control group. Comparing with control group, the heart rate of rabbit model injected HCN2 transfected mesenchymal stem cells doesn't change obviously.ConclusionComparing with formaldehyde chemical ablation, catheter ablation is a much better method to generate rabbit model of sick sinus syndrome. The right atrium of rabbit is not a suitable injection site for generation of biological pacemaker. |
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